高原环境下兔眼球挫伤后视网膜脑红蛋白的表达调控研究

Regulation of NGB in rabbit retina after eye contusion in high altitude

目的研究高原环境下兔眼球挫伤后视网膜脑红蛋白（NGB）的表达水平调控，探讨高原环境下眼球挫伤的损伤修复机制及救治。方法在高原环境用重击法造成2月龄健康北京青紫蓝兔眼球挫伤，随机分为吸氧组和非吸氧组，吸氧组动物模型在自制氧箱中以35％氧浓度每天持续给氧10h，于伤后12h、1d．3d,7d、14d分别摘取眼球，40g/L多聚甲醛固定．石蜡包埋切片，免疫组化检测NGB，比较吸氧组和非吸氧组NGB表达水平的不同。结果高原环境下兔眼球钝挫伤后吸氧组较非吸氧组NGB表达低，在3d和7d时差异有统计学意义（P〈0．05）。结论高原环境下兔眼球挫伤后通过吸氧可调控视网膜NGB的表达水平，伤后1d内视网膜处于应激期，吸氧等措施可减轻应激损伤，1～14d吸氧可大大促进视网膜损伤修复。

Objective To study the regulation of NGB in rabbit retina after eye contusion in high altitude. Methods The eye contusions were made by falling strike to the two-month-old rabbits in high altitude. Rabbits were divided into two groups at random： oxygen group and non oxygen group. The contused eyes were respectively extracted after 12 hours, 1 day, 3 days, 7 days and 14 days. The expression of NGB in the retina of two groups was examined by a sensitive immunohistochemical ABC method. Results The expression of NGB in the retinas of oxygen-supplying group was lower than that in non-oxygen-supplying group at the two time points--the 3rd day and the 7th day （ P 〈 0.05 ）. Conclusion These findings suggest that oxygen would regulate the expression of NGB in rabbit retina after eye contusion in high altitude. Oxygen can release the retina injury slightly before the 1 st day and encourage the recovery of retinal impair greatly between the 1st day to the 14th day.