胃癌组织中Treg的分布数量及其与IL-10、TGF-β关系研究被引量：5

Distribution quantity of Treg and relationship with IL-10 and TGF-β in gastric carcinoma tissue

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摘要目的研究CD4+CD25+调节性T细胞(Treg)在胃癌组织中的分布数量及意义。方法采用流式细胞术检测胃癌组织及正常组织中Treg数量变化;同时用免疫磁珠细胞分选技术分选出高纯度的CD4+CD25+T细胞,经体外刺激后检测胞内白介素-10(IL-10)和转化生长因子-β(TGF-β)分泌水平。结果正常组织中Treg数量为(8.40±0.52)%,胃癌组织中Treg数量明显增高〔(11.51±0.64)%〕。经体外刺激后,胃癌组织中Treg分泌的抑制性细胞因子(IL-10、TGF-β)较正常组织明显增高,差异有统计学意义(P<0.05)。结论Treg可能通过分泌IL-10和TGF-β等抑制性细胞因子干扰抗体抗胃癌的免疫反应。 Objective To study the distribution quantity and significance of CD4^＋CD25^＋ regulatory T cells（Treg）in gastric carcinoma tissue.Methods The quantity of Treg was determined by flow cytometry from gastric carcinoma tissue and normal tissue.Highly purified CD4＋CD25＋ T cells were isolated by immunomagnetic beads,and the production of intracellular suppressor cytokines such as interleukin-10（IL-10）and transforming growth factor-β（TGF-β）was tested by flow cytometry.Results The number of Treg in gastric carcinoma tissue was more than that in normal tissue.Compared with gastric carcinoma tissue,the secretion of intracellular cytokines such as IL-10 and TGF-β was significantly greater in peripheral CD4^＋CD25^＋T cells of normal tissue after in stimulation vitro（P〈0.05）.Conclusion Treg may obstruct the immunologic reaction of gastric carcinoma by secreting suppressive cytokines,and it plays an important role in gastric carcinoma prognosis.

作者胡宏波贾安平梁秀兰刘振鹏杨建詹前美

机构地区柳州铁路中心医院检验科柳州铁路中心医院消化内科

出处《重庆医学》 CAS CSCD 北大核心 2010年第3期265-267,共3页 Chongqing medicine

基金广西壮族自治区卫生厅科研基金资助项目(Z2008335 Z2009225)

关键词胃癌 T淋巴细胞白介素-10 转化生长因子-Β gastric carcinoma T-lymphocytes IL-10 TGF-β

分类号 R735.2 [医药卫生—肿瘤] R730.2 [医药卫生—肿瘤]

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参考文献17

1Sakaguchi S,Sakaguchi N. Regulatory T cells in immunologic self tolerance and autoimmune disease[J]. Int Rev Immunol, 2005,24 ( 3- 4 ) : 211.
2Beyer M, Schultze JL. Regulatory T cells in cancer[J]. Blood,2006,108(3) :804.
3Ohmura Y,Yoshikawa K,Saga S, et al. Combinations of tumoPspecific CD8+ CTLs and anti-CD25 mAb provide improved immunolherapy[J]. Oncol Rep, 2008, 19(5): 1265.
4Zaccone P, Burton O, Miller N, et al. Schistosoma manso ni egg antigens induce Treg that participate in diabetes prevention in NOD mice[J]. Eur J Immunol,2009,39(4) :1098.
5Nishikawa H, Kato T, Tawara I, et al. Accelerated chemi cally induced tumor development mediated by CD4+ CD25+ regulatory T cells in wild type hosts[J]. Proc Natl Acad Sci USA,2005,102(26) :9253.
6曾冬竹,余佩武,雷晓,石彦,王自强,郑峻松.CD4^＋ CD25^＋调控T细胞对小鼠胃癌的影响[J].中华胃肠外科杂志,2007,10(4):368-371. 被引量：7
7Thornton AM,Piccirillo CA,Shevach EM. ActiVation requirements for the induction of CD4+CD25+T cell sup pressor function[J]. Eur J Immunol, 2004,34 (2) : 366.
8Banerjee DK, Dhodapkar MV, Matayeva E, et al. Expansion of FOXP3 high regulatory T cells by human dendritic cells (DCs) in vitro and after injection of cytokine-matured DCs in myeloma patients[J]. Blood, 2006,108(8) :2655.
9Larmonier N, Marron M, Zeng Y, et al. Tumor-derived CD4 ( +) CD25 (+ ) regulatory T cell suppression of dendritic cell function involves TGF beta and IL-10[J]. Cancer Immunol Immunother, 2007,56 (1) : 48.
10Shevach EM, Dipaolo RA, Andersson J, et al. The - tyle of naturally occurring CD4+ CD25+ Foxp3+ regulatory T cells[J]. Immunol Rev, 2006,212 : 60.

二级参考文献15

1张乐萍,殷慧君,毛宁,周生.IL－2治疗微小残留白血病的动物实验研究[J].北京医科大学学报,1994,26(6):455-456. 被引量：2
2雷晓,余佩武,赵永亮,石彦,饶芸,曾冬竹,吴淼,周立新.CD4^+CD25^+调节性T细胞在胃癌患者的表达及其临床意义[J].第三军医大学学报,2006,28(5):397-400. 被引量：9
3曾冬竹,郑峻松,王自强,吴淼,余佩武.体外富集Treg细胞对CTL杀伤MNK45胃癌细胞活性的影响及机制研究[J].消化外科,2006,5(3):197-200. 被引量：2
4张惠铭付太顺邢德利等.L615小鼠脑膜白血病模型的建立和发病机制的初步探讨[J].中华血液学杂志,1983,4:335-339.
5Anna maria wolf,Dominik wolf,Michael steurer,et al.Increase of Regulatory T Cells in the Peripheral blood of cancer patients[J].Clin Cancer Res,2003,9(4):606
6Ermann J,Szanya V,Ford GS,et al.CD4+ CD25+ T cells facilitate the induction of T cell anergy[J].J Immunol,2001,167(8):4271
7Papiernik M.Natural CD4+ CD25+ regulatory T cells.Their role in the control of superantigen responses[J].Immunol Rev,2001,182(7):180
8Tanchot C,Vasseur F,Pontous C,et al.Immune regulation by self-reactive T cells is antigen specific[J].J Immunol,2004,172(4):4285
9朱立平,陈学清主编.免疫学常用实验方法[M].北京:人民军医出版社,1999.202
10Seddon B, Mason D. The third function of the thymus. Immunol Today, 2000,21 : 95-99.