茶多酚对ACC-M细胞株Fas、Fasl表达的影响

Effect of tea polyphenols on ACC-M cell Fas/Fasl expression

目的体外观察茶多酚对肺高转移性腺样囊性癌细胞株(ACC-M)生长、Fas及其配体Fasl表达的影响。方法(1)采用MTT比色实验法观察茶多酚对ACC-M细胞增殖的影响;(2)用免疫组化(SP法)检测不同浓度茶多酚对ACC-M细胞中Fas及其配体Fasl表达的影响。结果(1)茶多酚对ACC-M细胞增殖抑制作用明显,差异有统计学意义(P<0.05);(2)细胞免疫组化结果显示,加入不同浓度茶多酚后,Fas蛋白在ACC-M细胞中表达增高,差异有统计学意义(P<0.05),Fasl表达降低,差异有统计学意义(P<0.05),且随浓度增加更为明显。结论茶多酚可影响ACC-M细胞中Fas、Fasl表达,茶多酚具有抑制ACC-M细胞生长的作用可能与此有关。

Objective To observe the influence of polyphenols（TP）on the proliferation and analyze the expression of Fas/Fasl protein of adenoid cystic carcinoma of salⅣary gland cell（ACC-M）in vitro.Methods（1）The influence of TP on the proliferation of ACC-M was measured by MTT.（2）The expression of Fas/Fasl protein by streptavidin peroxidase method,and quantified measured by image analysis systems.Results（1）The more and longer use of TP on ACC-M,the higher restrain rate on the proliferation of ACC-M was observed（P〈0.05）.（2）The expression of Fas increased but Fasl declined after stimulated by TP by immunocytochemistry.It became distinct with the increase of TP concentration.Conclusion TP can inhibit the proliferation of ACC-M in vitro,and the mechanism has a correlation with induction of the expression of Fas,and down-regulation of the expression of Fasl.