人肺腺癌裸鼠皮下移植瘤模型的建立

Establishment of nude mouse transplantable model of human adenocarcinoma of lung by subcutaneous injection of SPC-A-1 cell line

目的采用人肺腺癌SPC-A-1细胞株建立裸鼠皮下移植瘤模型,为体内研究肺腺癌发病机制及靶向治疗提供工具。方法通过直接皮下注射细胞悬液法建立裸鼠皮下移植瘤模型,以每只2×106SPC-A-1细胞数接种于5只裸鼠右前肢腋窝皮下,观察成瘤时间、移植瘤长径、短径、重量、体积及病理形态等情况。结果5只裸鼠均成瘤,平均成瘤时间为7d,28d后瘤体平均长径为(19.20±3.85)mm,平均短径为(14.94±2.78)mm,平均瘤体体积为(2144.10±1237.72)mm3,平均瘤重为(1.37±0.60)g。镜下见大多数移植瘤包膜完整,瘤细胞腺样排列,呈腺癌形态,部分瘤块中央还可见局灶性坏死。结论成功建立裸鼠皮下人SPC-A-1肺腺癌模型,有利于后续体内实验。

Objective To establish a nude mouse model by subcutaneous injection of SPC-A-1 cell line, it was a useful tool for the in vivo research of lung adenocareinoma. Methods 2 × 10^6 SPC-A-1 cell suspention was subcutaneously injected into the armpit of five BALB/e nude mice in each mouse, then the formation of transplanted tumors was studied, its glowth velocity, weight of transplanted tumors and the morphous were closely observed. Results Transplanted tumors were formed in all nude mice. The average time of their formation was about 7 days. The average long diameter and short diameter of these tumors were about （19. 20±3.85） mm and （14. 94±2.78） mm respectively 28 days later, and the average volume of them was calculated to be about （2 144. 10± 1 237.72） mm^3. The average weight was calculated to be about （1. 37±0.6） g. Most transplanted tumors showed a continuous membrane, the tumor cells showed a same morphology of adenocarcinoma cells, and focal necrsis was found in some transplanted tumors by microscope. Conclusion The nude mouse model were successfully established by subcutaneous injection of SPC-A-1 cell line, it would provide a useful tool in the following research in vivo.