摘要
探讨中华补血草水提物(LSE)对抗N-乙酰对氨基酚(N-acetyl-p-aminophenol,APAP)引起的肝细胞损伤的作用及其可能的线粒体机制.通过分组实验,测定血清中谷丙、谷草转氨酶(sALT,sAST)的活性和谷胱甘肽(GSH)的含量,同时制备和观察肝组织切片.其后进一步探讨线粒体在这一过程中所发生的变化,通过测定线粒体的肿胀、线粒体膜电位(MMP)的变化以及电压依赖性离子通道(VDAC)在转录水平的变化等,探讨其相应的线粒体作用机制.结果显示:100、200、400mg/kg的LSE可以剂量依赖性地对抗APAP引起的肝细胞损伤,而这种护肝活性可能与LSE对肝细胞线粒体的保护作用有关.同时还发现,线粒体的一个重要的外膜蛋白——VDAC的表达可能与之有着密切的关系.
The present study was undertaken to investigate the hepatoprotective activity of aqueous extracts of Limonium sinense （Girard） Ktze root （LSE） against acetaminophen （APAP）-induced liver damage and to elucidate the possible mechanisms underlying the hepatoprotection. The serum alanine aminotransferase （sALT）,serum aspartate aminotransferase （sAST） activity and serum glutathione （GSH） content were detected,and the histopathology of liver was observed. The mitochondrial swelling,mitochondrial membrane potential （MMP） and transcription of voltage-dependent anion channels （VDAC） gene were also investigated. It was found that 100,200 and 400 mg/kg LSE could restore the APAP-induced changes on mice liver in a dose-dependent manner. And the mechanisms underlying its hepatoprotection might be related to the protection on liver mitochondria,especially on VDAC,the most important protein on outer membrane of mitochondria.
出处
《南京大学学报（自然科学版）》
CAS
CSCD
北大核心
2010年第1期100-107,共8页
Journal of Nanjing University（Natural Science）
基金
The Natural Science Foundation of Education(07KJA18017)
the Natural Science Research Foundation of Jiangsu Province ( 08KJB360011 )
the “Qinglan Project” Foundation for Young Core Teacher of Jiangsu Province
关键词
中华补血草
对乙酰氨基酚
肝保护作用
线粒体
电压依赖性离子通道
Limonium sinense （Girard） Ktze
acetaminophen
hepatoprotection
mitochondria
voltage-dependent anion channels
