海藻酸钠-多聚赖氨酸-海藻酸钠微囊对骨髓间充质干细胞生长分化性状的影响

The effect of APA microcapsule on MSCs growth and differentiation

目的探讨运用海藻酸钠-多聚赖氨酸-海藻酸钠(APA)微囊化免疫隔离技术对骨髓间充质干细胞(MSCs)生长分化的影响。方法采用脉冲式高压静电微囊制备仪制备出APA-MSCs微囊复合体,在成囊后第1天和第4天运用荧光素双醋酸酯/溴化乙锭(FDA/EB)染色确定MSCs成活率。采用离心破囊方法破囊,培养并观察破囊后MSCs形态和成骨作用等。结果微囊化后细胞在其内存活率约70%～80%;破囊后的细胞有活跃的增殖能力,经茜素红染色证实具有成骨潜力。结论MSCs在APA微囊内生长良好,保持干细胞特有生长分化,APA微囊可用于对MSCs进行免疫隔离。

Objective To study the effect of sodium alginate-poly-L-lysine-sodium alginate （APA） microencapsulated immunoisolation technology on the growth characteristic and differentiation of bone marrow-derived mesenchymal stem cells （ MSCs ）. Methods The APA-MSCs was made by the pulsed high-voltage electrostatic microcapsule instrument. The MSCs＇ Survival rate in the APA microcapsule was determined by the fluorescein diacetate/thidium bromide （FDA/EB） double staining. The capsules were broken by the centrifuge. And then the MSCs were ellected, cultured＇ and observed their shape and potential of bone formation. Results The survival rate of the MSCs in APA mieroeapsule was about 70% - 80%. The MSCs out of APA microcapsule showed active proliferation capacity in vitro and its bone formation potential was confirmed by alizarin red staining. Conclusions The MSCs has a good survival in APA microcapsules. Their differentiation is maintained. APA mierocapsule could he used for immunoisolation of MSCs.