冠心病患者外周血单个核细胞TLR5的测定及临床意义

Increased expression of Toll-like receptor 5 in peripheral blood mononuclear cells in patients with coronary arteriosclerosis disease

目的:检测Toll样受体5(Toll-like receptor5,TLR5)在冠心病(CHD)患者外周血单个核细胞(PBMCs)的表达水平,并探讨其与冠状动脉粥样硬化发生发展的关系。方法:用逆转录聚合酶链扩增法(RT-PCR)的方法检测50例CHD患者及40例健康对照者PBMCsTLR5 mRNA的表达情况。结果:CHD患者外周血单个核细胞Toll样受体5mRNA表达水平高于健康对照组,分别为(0.793±0.284)和(0.211±0.319)(P<0.01)。结论:TLR5及其介导的天然免疫应答与动脉粥样硬化的发生发展存在一定的相关性。

Objective：To investigate the expression of Toll-like receptor5（TLR5） in peripheral blood mononuclear cells（PBMCs ）in the patients with coronary arteriosclerosis disease（CAD ）,and to explore the relationship between TLR5 expression and coronary arteriosclerosis.Methods：TLR5 mRNA levels were respectively measured by reverse transcription-polymerase chain reaction（RT-PCR） in 50 CAD patients and 40 healthy controls.And the levels of plasma lipids were determined by automatic biochemistry analyzer.Results：The mean TLR5 mRNA numbers in CAD group were significantly higher than in healthy controls 0.793±0.284 vs 0.211±0.319（P〈0.01）,but no significant difference was found between CAD patients with normal and abnormal levels of plasma lipids.Conclusion：The expression is increased in the patients with coronary arteriosclerosis disease.TLR5 and TLR5-mediated natural immune responses are correlated with the development and progression of arteriosclerosis.