电针复合静松灵麻醉对山羊痛阈及生理生化的影响

Effects of Electroacupuncture Anesthesia Combined with Xylidinothiazoline on Pain Threshold and Physiological and Biochemical Indexes in Goats

【目的】了解电针复合静松灵麻醉对山羊麻醉痛阈值及生理生化指标的影响。【方法】30只成年杂交山羊,体重22—27kg,随机分为0.5mg·kg-1静松灵组、2mg·kg-1静松灵组、电针复合0.5mg·kg-1静松灵组、电针组和空白对照组,每组6只,公母各半。分别在给药后0、5、25、45、65和85min(单纯药物组),或电针后0、25、45、65、85、105min(电针组或电针复合药物组)采用钾离子透入法测定痛阈值,并测定呼吸频率、心率、平均股动脉压、体温等生理指标,于试验前及试验后1.5、24、48、96和168h,采集山羊血液分别测定丙氨酸氨基转移酶活性、天门冬氨酸氨基转移酶活性、血清尿素含量、血糖含量、皮质醇含量等指标。【结果】电针复合0.5mg·kg-1静松灵组山羊的痛阈在给药后显著高于单纯电针组和0.5mg·kg-1静松灵组(P<0.05),而与2mg·kg-1静松灵组无显著性差异(P>0.05);电针复合0.5mg·kg-1静松灵组山羊的呼吸频率、平均股动脉压、天门冬氨酸氨基转移酶活性以及血清葡萄糖、尿素和皮质醇含量与对照组无显著差异(P>0.05),而0.5mg·kg-1静松灵组与2mg·kg-1静松灵组山羊天门冬氨酸氨基转移酶活性及血清葡萄糖、尿素或皮质醇含量与对照组差异显著(P<0.05);电针复合0.5mg·kg-1静松灵对山羊的心率、体温和丙氨酸氨基转移酶活性的影响显著低于0.5mg·kg-1或2mg·kg-1静松灵(P<0.05)。【结论】山羊电针复合静松灵麻醉痛阈值高,麻醉效果较好,生理生化指标干扰小。

[ Objective ] The physiological and biochemical effect of electroacupuncture anesthesia combined with xylidinothiazoline in goats was studied in this paper. [Method] Thirty adult hybrid goats, weighting 22-27 kg, were divided into 0.5 mg·kg^-1 xylidinothiazoline group, 2 mg·kg^-1xylidinothiazoline group, electroacupuncture combined with 0.5 mg·kg^-1 xylidinothiazoline group, electroacupuncture group and control group. Each group had six goats, which were equally divided between male and female. At 0, 5, 25, 45, 65 and 85 min after the goats were gaven injection of xylidinothiazoline （0.5 mg·kg^-1 xylidinothiazoline group and 2 mg·kg^-1 xylidinothiazoline group） or at 0, 25, 45, 65, 85 and 105 rain after the goats were subjected to electroacupuncture （electroacupuncture group or electroacupuncture combined with 0.5 mg·kg^-1 xylidinothiazoline group） the iontophoresis of potassium was used to observe the pain threshold. At the same time, heart rate, mean arterial blood pressure, respiratory rate, body temperature and other physiological indicators were observed. Before the experiment and at 1.5, 24, 48, 96 and 168 h after the experiment, the goat serum was collected for measuring alamine aminotransferase and aspartate aminotransferase activities, and concentrations of urea, blood sugar and cortisol. [Result] The pain threshold of goats underwent electroacupunctureplus xylidinothiazoline at 0.5 mg·kg^-1 was higher than that of goats administered xylidinothiazoline at 0.5 mg·kg^-1 or goats received electro-acupuncture alone （P〈0.05）, and had no significant difference from that of goats given xylidinothiazoline at 2 mg·kg^-1 （P〉 0.05）. Respiratory rate, mean arterial blood pressure, aspartate aminotransferase activity, and concentrations of serous glucose, urea and cortisol in goats that received electroacupuncture combined with xylidinothiazoline at 0.5 mg·kg^-1 were not different （P〉0.05） from those in the control goats, while aspartate aminotransferase activity, and concentrations of glucose, urea and cortisol in goats given xylidinothiazoline at 0.5 mg·kg^-1 or 2 mg·kg^-1 alone were different （P〈0.05） from those in the controls. Electroacupuncture plus xylidinothiazoline at 0.5 mg·kg^-1 affected heart rate, body temperature and alamine aminotransferase activity less than （P〈0.05） xylidinothiazoline administration at 0.5 mg·kg^-1 or 2 mg·kg^-1 alone did. [Conclusion] Electroacupuncture combined with xylidinothiazoline is better for goat anesthesia and has small interference to the goat physiological and biochemical indicators.