摘要
目的:建立Folin比色法测定鹿衔草提取物中总多酚的含量。方法:采用分光光度法测定。以没食子酸为指标,检测波长730 nm。结果:没食子酸在1.08μg/mL^6.48μg/mL范围内线性良好,相关系数为0.9999,加样回收率99.7%,RSD为1.2%(n=9)。结论:本法测定鹿衔草提取物中总多酚的含量,结果准确可行。
Objective：To establish a special determination method of polyphenol ingredient in Pyrola extract. Methods： The content of total polyphenol was detected by chromatometry using gallic acid as index at the wavelength of 730 nm. Results ： The lin- ear range of calibration curve was 1.08μg/mL - 6.48 μg/mL for the gallic acid （ r = 0.9999）. The average recovery was 99.7% and RSD was 1.2%（ n = 9）. Conclusion： The method is accurate, can be used in the quality control of Pyrola callitha H.Andres extract.
出处
《长治医学院学报》
2010年第1期8-10,共3页
Journal of Changzhi Medical College
关键词
总多酚
鹿衔草
Folin比色法
含量测定
Total polyphenol
Pyrola callitha H. Andres
Folin colorimetry
Content determination
