利用RAPD技术鉴别玉米自交系

Identification of Maize Inbred Lines with Random Amplified Polymorphic DNA(RAPD)

本文利用ＲＡＰＤ技术对我国２８个玉米骨干自交系进行了分析，从３００个引物中筛选出了４０个多态性好的引物，结果发现：仅使用ＯＰＩ１０一个引物扩增的ＤＮＡ指纹图谱就能区别开１５个自交系，增加引物ＯＰＢ７可以区分开２６个自交系，再增加ＯＰＣ１９、ＯＰＧ６、ＯＰＡ７、ＯＰＩ１１、ＯＰＧ１４中的任何一个引物就可以将２８个自交系完全区分开。研究还表明亲缘关系很近、利用同工酶方法无法区分的姊妹系材料如：４７８、４８８和３１８９，以及双１０５和双７４１都能够被明显的区分开，证明了利用ＲＡＰＤ技术进行玉米自交系鉴定完全可行而且是非常有效的。

main maize inbred lines were analysed by using RAPD technology.40 primers which amplified polymorphic bands were screened from 300 primers.The results illustrated that 15 inbreds were identified using the primer OPI10,and 26 inbred lines could be distinguished by adding primer OPB07.If any of the primers OPC19,OPC6,OPA7,OPG14 Was added,28 inbreds were able to be completely identified.The experiment also showed that the close relative inbred lines such as 478,488with 3189,and Shuang 105 with shuang 741,which could not be distinguished by isoenzyme,could be identified obviously using RAPDs.The study demonstrated that it was practical and effective to identify maize inbreds with RAPDs.