蒙古族原发性高血压人群ACE I/D、AGT M235T及CYP11B2 T-344C多态性环境与遗传交互影响的联合分析

A associativity study on environment,inheritance and gene ploymorphisms of ACE I/D,AGT M235T,CYP11B2 C-344T in Mongolia essential hypertension patients.

目的我们将对内蒙古蒙古族人群的ACE基因插入/缺失(I/D)多态性和CYP11B2基因启动子区C-344T、AGTM235T多态性及环境因素进行综合分析,探讨蒙古族人群患原发性高血压的遗传危险因素。方法应用PCR-RFLP技术检测164例原发性高血压患者与正常对照组109名健康受试者ACE基因I/D、AGT基因M235T、CYP11B2基因C-344T多态性。结果(1)高血压病组和对照组的临床特征经比较采用logistic多元回归分析结果为两组资料在年龄、饮酒、高血压家族史方面存在统计学差异(P分别为0.013,0.006,0.005),年龄、饮酒及具有高血压家族史者发病风险增加1.031,2.122和2.399,95%可信区间为1.006-1.056,1.238-3.637和1.306-4.405。(2)两组基因型及等位基因频率比较,3个基因位点基因型及等位基因频率无统计学差异(P>0.05)。(3)两组之间采用logistic回归分析结果为CYP11B2位点CT基因型的发病风险为0.592,P值为0.047,χ2值为3.929,95%可信区间为0.353-0.994,其他基因型结果均为P>0.05。结论年龄、饮酒及具有高血压家族史在蒙古族高血压的发病过程中具有交互作用;CYP11B2位点CT基因型与其它位点存在交互作用,可能是高血压的遗传保护因素。

Objective：To investigate Mongolia essential hypertension patients heredity risk factor by aggregate analysis of gene ploymorphisms of ACE I/D,CYP11B2 C-344T,AGT M235T and environmental factor. Methods：PCR-RFLP technique was used to test gene ploymorphisms of ACE I/D,AGT M235T,CYP11B2 C-344T of 164 Mongolia essential hypertension patients and 109 Mongolia healthy controls.Results：（1）Multiple regression analysis show age、 Drinking and hypertension family history aspects have statistics difference between two groups P=（ 0.0132,0.006 and 0.005）,OR =（1.031,2.122 and 2.399）,95%CI （1.006-1.056,1.238-3.637 and 1.306-4.405）.（2）We compared genotype and the distribution of allele frequencies between case and control groups,There weren′t significant difference （P〉0.05）. （3）CT genetype of CYP11B2 comparing between two groups ,logistic gradual regressive analysis shows P=0.047,OR= 0.592,χ^2=3.929,95%CI（ 0.353-0.994）,the other genetypefinding compared shows P〉0.05.Conclusion：age,Drinking and hypertension family history have interaction in Mongolia essential hypertension onset process ,CT genetype of CYP11B2 exist interaction with other gene site,It may be a heredity protection factor in essential hypertension.