宫颈上皮内瘤样病变患者高危型HPV感染基因分型分析

The analysis of the infection and genotyping of the high-risk HPV in CIN patients

目的了解宫颈上皮内瘤变患者的高危型HPV的感染及其分型和不同程度宫颈病变的主要感染型别情况。方法应用型特异PCR检测宫颈癌前病变的患者的主要高危型HPV-16、18、33、58的感染及其分型情况的相关性研究。结果在本研究宫颈上皮内瘤变患者98例中,4种高危型HPV的总阳性例数为73例,HPV总的感染率为74.5%,存在多重感染。其中HPV-16、18、33、58的总感染率分别为53.1%、38.7%、17.3%和30.6%。CIN的Ⅰ/Ⅱ/Ⅲ3组患者的4种高危型HPV的感染率分别为42.9%、61.1%和93.2%。结论主要高危型HPV在宫颈上皮内瘤变患者中感染的主要型别依次为HPV16、HPV18、HPV58、HPV33,主要为HPV16和HPV18型;不同程度CIN的高危型HPV的总感染率不同,随病变程度的加重而增加。

Objective：To study the main HPV infection types and genotyping of the CIN patients. To study the prevalent HPV types in different grade of CIN. Methods：Type-specific PCR was used to detect HPV-16,18,33,58 and genotyping of cervical intraepithelial neoplasia （CIN）. Results：There were 73 samples screened for the presence of HPV-DNA form 98 patients. The positive rate of total HPV was 74.5%. The infection rates of the four HPV types were as follows：HPV16 （53.1%）,HPV18 （38.7%）,HPV33 （17.3%）,HPV58 （30.6%）. The Positive rates of total HPV in different grade of CIN were 42.9%,61.1% and 93.2% respectively. Conclusion：The prevalent HPV types of CIN from highest to lowest order were as follows：HPV16,HPV18,HPV58 and HPV33. The positive rates of total HPV in different grade of CIN were different,increasing with the seriousness of cervical neoplasia lesions.