生姜透皮帖对运动病大鼠脑内酶学变化的作用

Effect of ginger transderm scopolamine on changes of enzymes activity in brain tissue of rat suffered from motion sickness

目的：建立运动病模型，观察生姜透皮帖对运动病大鼠脑组织几种生化指标的变化，并探讨其对运动病产生治疗作用的机制。方法：雄性Wistar大鼠40只，随机分5组，每组8只，每天按实验方法刺激，实验第4天刺激结束后取大脑和小脑皮质、脑干前庭区。采用组织化学定位方法和图像定量分析方法，检测各组大鼠3部位脑组织中碱性磷酸酶（ALP）、K^＋Na^＋ -ATP酶及细胞色素氧化酶（CCO）活性。结果：运动病大鼠3部位脑组织中ALP活性显著升高、NatKtATP酶及CCO活性明显减低。使用生姜透皮帖后运动病大鼠3部位脑组织中ALP活性显著减低、Na^＋-K^＋-ATP酶及CCO活性明显升高。结论：生姜透皮帖可能使脑血管紧张度降低，改善脑血流的分布，增加脑血流，调整脑细胞内CCO、ALP、Na＋-K＋ATP酶的活性，增强脑细胞的兴奋性，从而保持中枢神经系统的兴奋性，起到抗运动病的作用。

Objective： To observe the biochemical changes in the brain tissue of rat suffering from motion sickness, thereafter to explore its mechanism. Methods： 40 SD rats were randomly and equally divided into five groups, including a normal group and four model groups, of which three groups were treated with different dose of ginger transerm scopolamine. After motion stimulation for four consecutive days, the cerebral cortex, cerebellar cortex and vestibular area of brain stem in rats were removed to detect activity of the alkaline phosphatase, Na＋-K＋- ATPase and cytochrome oxidase by means of histochemical techniques and image analysis. Results： Motion sickness induced a significant increase in the alkaline phosphatase activity in the examined rat brain tissues, which was suppressed by ginger transderm scopolamine. The activities of Na＋-K＋ ATPase and cytochrome oxidase were decreased obviously in the rat brain tissues after motion sickness was duplicated, but were improved significantly the by ginger transderm scopolamine. Conclusion： Ginger transderm scopolamine against motion sick- ness might attribute to its capability for inhibiting vascular tone, improving the distribution of blood flow and increasing cer- ebral blood flow which could relieve partial blood supply, hippocampal hypoxia and scavenge free radicals.