西藏小型猪细胞色素b基因序列的比较

Comparison of the Cyt b Gene Sequences in Tibet Mini-Pig and Other Chinese Pigs

目的对西藏小型猪Cyt b-基因序列进行分析,研究其遗传背景及其与国内家猪的亲缘关系。方法提取西藏小型猪和巴马小型猪、贵州香猪、五指山猪的全基因组DNA,设计引物扩增Cyt b基因,测序后进行碱基序列比对,建立亲缘关系树,分析西藏小型猪的遗传背景。结果西藏小型猪等国内部分品种猪的Cyt b基因序列与欧洲猪相比有14个变异位点;但是西藏小型猪与国内品种猪相比存在两个特殊碱基位点即在420位点T→C转换的同时在883位点存在G→A转换。结论西藏小型猪与巴马小型猪、贵州香猪、五指山猪等国内家猪有很近的亲缘关系。同时进一步证实西藏小型猪群体内存在一定的遗传分化。

Objective To analyze the Cyt b gene sequences in Tibet mini-pigs and clarify the differences and genetic relationship with other Chinese pigs. Method The sequence of Cyt b gene was amplified from genome DNA of Tibet mini-pig,Bama miniature pigs,Guizhou xiang pigs and Wuzhishan（WZS） pigs.After sequencing,the base sequences were compared and analysed.The blood relationship tree and evolution position of Tibet mini-pig were established. Result There were 14 mutation sites between domestic pigs in China and pigs from Europe.Besides there was a significant difference in two nucleotide site ： a T→C switch in site 420 and the G→A switch in site 883 at the same time.Conclusion Chinese pigs include Bama miniature pigs, Guizhou xiang pigs and WZS pigs,have a very close blood relationship with some of Tibet mini-pigs.It has been confirmed that there is a certain genetic differentiation in the Tibet mini-pig.