尾悬吊大鼠比目鱼肌‘肌肉生长抑制素’基因的表达水平

Myostatin Gene Expression Level in the Soleus Muscle of Tail-Suspended Rats

目的确定尾悬吊大鼠比目鱼肌组织中‘肌肉生长抑制素’(myostatin,MSTN)基因的表达水平。方法在测定尾悬吊14、30 d大鼠后肢比目鱼肌相对湿重的基础上,采用real-time RT-PCR方法,在基因水平对尾悬吊14、30 d大鼠比目鱼肌中MSTN mRNA水平进行检测。结果尾悬吊14、30 d后比目鱼肌的相对湿重分别下降了11%和19%。real-time RT-PCR结果表明,大鼠尾悬吊14、30 d后,比目鱼肌中MSTN mRNA表达水平均有所提高,分别是对照大鼠的2.2倍和3.5倍。结论尾悬吊大鼠后肢去负荷后可诱导抗重力肌-比目鱼肌组织中MSTN mRNA表达水平的上调。

Objective To study the effect of tail-suspending on myostatin（MSTN） expression in the soleus muscle of rats.Methods The MSTN mRNA levels in soleus muscle of tail-suspended and normal control rats were measured and compared.Results The MSTN mRNA in soleus muscle of tail-suspended rats was 2.2 times（hind limb unloading for 14 days） and 3.5 times（hind limb unloading for 30 days） higher than that in normal control rats（P0.05）.The relative wet weight of the soleus muscle in tail-suspended rats was decreased by 11 %（unloading 14 days） and 19 %（unloading 30 days）,respectively,compared with that in normal control rats.Conclusion The expression of MSTN mRNA is elevated in the soleus muscle of tail-suspended rats,probably due to atrophy of the soleus muscle.