下颌牵张成骨中应用神经生长因子对神经损伤修复的作用

Recovery effect of systemic injection of nerve growth factor on inferior alveolar nerve in a rabbit model of mandibular distraction osteogenesis

目的研究下颌骨牵张成骨中全身注射神经生长因子(NGF)对损伤的下齿槽神经的修复作用。方法新西兰白兔48只,全麻下行双侧下颌骨牵张成骨术,术后第4天以0.5mm/12h的速度牵张,共10天。术后即分别给予肌注NGF0.6μg/d×20天和相当量生理盐水。采用BL-420F生物机能实验系统及检测设备,分别在术前、牵张期结束、固定期1、2、4周时行感觉神经动作电位(SNAP)测试。结果在牵张完成时、固定期1周,NGF组和对照组的SNAP波幅和潜伏期无统计学差异;在固定期2、4周,NGF组波幅明显高于对照组,而潜伏期明显低于对照组(P<0.05)。结论全身注射NGF有利于牵张成骨中下齿槽神经功能的恢复。

Objective To study the recovery effect of the inferior alveolar nerve following systemic injection of NGF （ nerve growth factor） in a rabbit model of mandibular distraction osteogenesis. Methods Totally 48 New Zealand white rabbits underwent bilateral distraction osteogenesis with a rate of 0.5mm/12h for 10 days. Immedi- ately after surgery,0.6μg/d × 20d NGF or normal saline were intramuscularly injected respectively. BL-420F moni- toring system was applied to record the sensory nerve action potentials（SNAP） before surgery, at the end of distrac- tion,or at consolidation time of 1,2,4 weeks respectively. Results At the end of distraction and 1 week following distraction,the amplitude of the evoked potentials and latency showed no statistical difference between the NGF group and control group ;at the consolidation time of 2,4 weeks, the amplitude of the evoked potentials and latency was significantly higher and lower respectively in NGF group than that in control group（ P 〈 0.05）. Conclusion Systematic injection of NGF can help to accelerate the function recovery of inferior alveolar nerve in mandibular dis- traction osteogenesis.