正交设计法优化三岛柴胡愈伤组织培养基

Optimization of Medium for Bupleurum.falcatum L. Callus by Uniform Design

目的:优化三岛柴胡愈伤组织培养条件。方法:采用正交设计法,以相对生长速率、鲜重和干重为考察指标,对三岛柴胡愈伤组织生长的MS培养基成分进行单因素多水平和多因素多水平考察。结果:最佳培养基为:MS+3%蔗糖+10/40mmol.L-1NH4+/NO3-+3.75mmol.L-1HPO24-+NAA(0.005mg.ml-1)+6-BA(0.010mg.ml-1)。最佳培养条件下,愈伤组织相对生长速率为0.1548,干重达0.1668g.瓶-1。结论:2,4-D与NAA配合使用可以促进三岛柴胡愈伤组织快速生长,L9(34)正交设计实验中愈伤组织干重明显高于单因子实验设计的。

Objective：To optimize the medium for Bupleurum. falcatum L. callus. Methods： The components of MS medium for growth of callus were investigated from single - factors and poly - levels and poly - factors and poly - levels by orthogonal design. And relative growth rate, fresh weight and dry weight was chosen as the experiment index. Result： The best optimum medium was MS added 3% sucrose, 10/40mmol.L^-1 NH4^＋/NO3^-, 3.75mmol.L^-1 HPO4^2- ,0. 005mg.ml^-1 NAA,0.010mg.ml^-1 6 - BA. The callus relative growth rate was 0. 1548, the dry weigllt was 0. 1668g. Conclusion：2,4 - D and the NAA coordination use might promote B.falcatum callus grow fast. The L9 （34） orthogonal design experiment had high dry weight than arrangements in single -factor design.