竹材木素降解菌发酵条件优化及竹材降解红外光谱分析

Optimum Condition of Selectively Decomposing Bamboo by the White Fungi Lignin and FTIR Analysis on Bamboo Lignocelluose Degradation

目的:对所筛选菌株进行发酵条件的优化,以期找到适合该菌的最佳培养条件。方法:通过单因素试验及正交试验,得到最佳培养条件,并且对降解后的竹材结构进行红外光谱分析。结果:得到最佳培养条件:培养温度35℃,初始pH5.5,摇瓶装量40mL,摇床转速160r/min时,该菌株具有较高的酶活力。Mnp和Lip酶活力分别达到631U/L和340U/L,比原发酵条件提高了45.9%、49.3%。红外光谱分析后发现竹材中的木质素结构被严重破坏,难降解的大分子长键烃被切断成易降解的小分子短键烃。结论:所筛选菌株对竹材木质素有选择性降解能力,确定其发酵条件后为进一步试验奠定基础。

Objective：In order to research the optimum culture condition of a white rot fungi Trametes versicolor CSUFT -00718, the fermentation conditions were investigated. Method ： The optimal culture conditions were obtain through the single - factor test and orthogonal test. Orthogonalhe structure of the untreated bamboo and the treated bamboo degraded under the optimal fermentation condition was analyzed through infrared spectroscopy. Result： The optimum culture components are： loading amount 40 mL, culture temperature 35℃, initial pH5.5 , rotational speed of 160r/min. The activity of Mnp and Lip reached 931U/L and 543 U/L after eight days. Compared with the formal femlentation condition, the enzyme activity enhanced 45.9% and 49.3%, respectively. Compared with the structure of untreated bamboo, the structure of the treated bamboo was greatly altered long - bond hydrocarbons of giant molecule diffictdt to be biodegraded were degraded into short - bond hydrocarbons of small molecule, which could be easily biodegraded. Conclusion： The strains could selectively decomposing bamboo lignin.