橡胶树死皮橡胶粒子膜蛋白差异分析与初步鉴定

Differential Analysis of 2-DE Profiles and Preliminary Identification of Lutoids Proteins from TPD Tree in Hevea brisiliensis

橡胶树死皮(tapping panel dryness,TPD)是制约天然橡胶生产的主要限制因子,在世界各橡胶种植园中普遍发生,给橡胶种植业带来严重的危害。目前仍无有效措施防治死皮发生,而橡胶树死皮发生分子机制是制定有效防治措施的前提。为阐明死皮发生的分子机制,应用双向凝胶电泳技术(2-DE),比较橡胶树死皮株与健康株橡胶粒子蛋白质组表达的差异。采用固相pH梯度双向凝胶电泳分离橡胶树死皮株与健康株橡胶粒子总蛋白质,经考染显色后,用PD Quest7.40图像分析软件进行比较分析,识别差异表达的蛋白质。差异点经过胶内酶切和基质辅助激光解析电离飞行时间质谱(MALDI-TOF-MS)分析获取肽质指纹图谱(PMF),Mascot软件搜索SWISS-PROT和NCBI nr数据库鉴定蛋白质。经过上述分析共鉴定出13个蛋白差异表达点,与健康株相比,在死皮中上调表达的蛋白质点有11个,下调表达的蛋白质点有2个,这些差异表达的蛋白质可能在橡胶树死皮发生和发展过程中发挥重要作用。

Tapping panel dryness （TPD） is one of the most serious threats to natural rubber proaucnon, it exmts in almost every rubber plantation country and causes serious damage to natural rubber production. It is well known that elucidation of molecular mechanism of TPD is prerequisite for taking effective measures to prevent TPD. To elucidate the molecular mechanism of TPD, the expression profiles of lutoids proteins from TPD and healthy rubber trees was analyzed by 2-DE method and PDQuest7.40 software. After digesting peptides differentially expressed with enzymes, the peptide mass fingerprints were obtained with MALDI-TOF-MS, and then identified by searching SWISS-PROT and NCBI nr database. In this research, a total of 13 peptides.