基于2-DE结合质谱研究围产期奶牛血浆蛋白表达的变化

Changes of Plasma Proteins in Transition Dairy Cows Based on 2-DE Combination with Mass Spectrometry

本试验旨在揭示奶牛围产期免疫功能下降的机理。采用二维凝胶电泳方法建立了分娩前第21天、分娩后第1和21天血浆蛋白表达图谱,SYPRO ruby染色后,ImageMaster 2D platinum 6.0软件匹配检测差异表达蛋白点,液相色谱串联质谱鉴定。结果表明:与分娩前第21天和分娩后第21天相比,分娩后第1天血浆中转甲状腺素蛋白表达量下调、而结合珠蛋白和α1酸性糖蛋白表达量上调;与分娩前第21天和分娩后第1天相比,分娩后第21天载脂蛋白A1表达量增加,这些表达变化的蛋白涉及急性期应答反应及运输和代谢等生物学功能。结果提示奶牛分娩后急性期蛋白表达量的增加可能与免疫功能下降相关。

The mechanism of immune system suppression was investigated in transition dairy cows.Changes of plasma proteins on the 21 d before calving,on the 1 d and 21 d after calving were separated by two-dimensional electrophoresis providing a platform for parallel analysis.After visualization proteins with SYPRO ruby,differentially expressed proteins were analyzed by ImageMaster 2D Platinum 6.0 software,and identified by HPLC equipped with ion trap mass spectrometer.The result showed that transthyretin was down-regulated on the 1 d after calving,while haptoglobin and alpha-1 acid glycoprotein were up-regulation,with a much larger and more abrupt variation at parturition,comparative with the 21 d prepartum and postpartum;the expression abundance of apolipoprotein A-Ⅰ increased on the 21 d postpartum compared to prepartum and at parturition.These proteins were involved in acute-phase response,transport and metabolism.The findings may provide valuable information for the exploring of the decreased immune function during the transition dairy cows.