不同禽源贝氏隐孢子虫分离株对鹌鹑致病性的比较研究

Pathogenicity of Cryptosporidium baileyi Isolates Derived from Different Poultry Hosts in Quail

本研究旨在观察不同禽源贝氏隐孢子虫（Cryptosporidium baileyi）对鹌鹑的致病性差异。采用光镜、扫描电镜等技术研究了鸡源、鸭源、鹌鹑源、白文鸟源、鸵鸟源贝氏隐孢子虫分离株对10日龄鹌鹑的致病性。结果表明,感染鹌鹑均出现腹泻和呼吸道症状,排卵囊持续期11~18 d,虫体寄生于喉头、气管、法氏囊和泄殖腔。其中鸭源C.baileyi感染组鹌鹑发病率和死亡率分别为82.5%、25%,鸡源C.baileyi（郑州株）感染组发病率和死亡率分别为77.5%、12.5%,鹌鹑源C.baileyi感染组发病率、死亡率分别为37.5%和17.5%。本研究结果提示,不同禽源C.baileyi对鹌鹑有一定致病性,其致病性强弱存在差异。

This experiment was conducted to explore the pathogenicity variance of Cryptosporidium baileyi isolates derived from different poultry hosts in quails.The chicken-derived,duck-derived,quail-derived,White Java sparrow-derived,and ostrich-derived C.baileyi isolates were inoculated to 10-day-old quails respectively and the pathogenicity was observed by microscopy and scanning electron microscopy（SEM） techniques.Diarrhea and the symptom of respiratory passage were shown,and the patent period of oocyst shedding was 11-18 days.The development stages were found to localize in the throat,trachea,bursa of Fabricius and cloacal chamber.In the duck-derived C.baileyi infected group,the morbidity and mortality were 82.5% and 25%,respectively.Likewise,the morbidity and mortality were 77.5% and 12.5% in Zhengzhou chicken-origin C.baileyi infected group,37.5% and 17.5% for the quail-origin infected group.The present results suggest that there was pathogenicity variance in quails using C.baileyi isolates derived from different hosts,and the intensity of pathogenicity variance was corresponding to different C.baileyi isolates.