西红花苷和西红花酸在小鼠体内抗氧化活性对比研究

Comparative evaluation of the antioxidant capacity of crocetin and crocin in vivo

目的对比评价西红花苷和西红花酸在小鼠体内抗氧化活性。方法采用硅胶柱层析从栀子中分离西红花酸和西红花苷纯品,普通昆明小鼠灌胃给药西红花酸和西红花苷,剂量分别是西红花酸6.25、12.5和25.0mg·kg-1和西红花苷18.7、37.5和75.0mg·kg-1.d-1,采用测定普通小鼠体内抗氧化指标(SOD、GSH-Px、TAOC和MDA)的方法对比评价西红花酸和西红花苷-1体内抗氧化活性。结果两种化合物都能明显提高小鼠肾脏和肝脏的SOD,肝脏的GSH-Px,心脏和肾脏的TAOC,降低血浆的MDA。结论西红花酸和西红花苷-1具有相似的体内抗氧化活性,其主要活性部位可能是肝脏和肾脏。

Aim To investigate the antioxidant capacity of crocetin and crocin in an in vivo system. MethodsColumn chromatography was applied to the seperation of crocetin and crocin-1 from gardenia. Crocetin（6.25, 12.5 and 25.0 mg · kg^-1·d^-1） and crocin （18.7, 37.5 and 75.0 mg · kg^-1 . d^-1 ） were orally administered to kunming mice. Then, superoxide dismutase （ SOD）, glutathione peroxidase （ GSH-Px）, total antioxidant capacity （ TAOC ） and malondialdehyde （MDA） in mice were determined for the comparison of antioxidant activity of crocetin and crocin-1. Results Oral admin- istration of crocetin and crocin for six weeks could en-hance SOD of liver and kidney, GSH-Px of liver and TAOC of heart and kidney. In addition, it could decrease MDA of serum in mice. Conclusions The comparison of results suggests the evidence supporting the comparable antioxidant activity of crocetin and crocin. The results of the research also indicate that liver and kidney are two organs targeted for protection concerning endogenous antioxidant among various tissues.