应用AFLP方法研究中国大豆的遗传多样性(英文)

Genetic diversity of Chinese soybean germplasm revealed by amplified fragment length polymorphism

本试验采用AFLP技术，研究了61份来自中国四个不同大豆生态区的栽培和野生大豆材料的遗传多样性。8对AFLP引物共检测到569条谱带，平均为71条。273条（48％）为多态性谱带，其中172条多态性谱带只出现在栽培大豆，249条则只属于野生大豆。不同的引物组合检测多态性谱带的能力有很大差异，变幅为17～51引条。Eaag／Mctc是十分高效的引物组合，通过该引物组合所有61份材料都能被区分。聚类分析能清楚地将栽培大豆与野生大豆区别开来，在某种程度上，也能鉴定出不同类型的栽培大豆，如春大豆。聚类分析还表明，我国黄淮海地区的大豆可能较其他地区具有较高水平的遗传多样性。

In this study, amplified fragment length polymorphism(AFLP) was used to evaluate genetic diversity among 61 Chinese soybean accessions from four different ecological regions (15 provinces) including 41 cultivated (Glycine max L.Merr.)and 20 wild soybean (Glycine soja Siebold & Zucc.). Eight AFLP primer pairs detected a total of 569 fragments with an average of 71 fragments per primer combination. Two hundred and seventy three fregments,representing 48% of total fragments, were polymorphic, in which 172 were present in G. maxand 249 were in C. soja. The primer combinations differ widely in their ability to detected polymorphism. The range of polymorphic detected by individual primer combination was from 16 to 51. Eaag/Mctc is particularly informative detecting 51 (62, 2% ) polymorphic fragments, which differentiated all 61 accessions. Cluster analysis based on unweighted pair-group method using arithmetic average (UPGMA) clearly separated the two species as present taxonomy does and,to some extent,the different sowing type of G.max, particularly the spring sowing type accessions in spite of their differences of geographical origin. Cluster analysis the suggested that accessions from of yellow river region may have a higher level of genetic diversity in respect to the germplasm collected in other regions.