中华鳖体液免疫应答的初步研究

Preliminary studies on humoral immune response of soft-shelled turtle

不同季节用嗜水性单胞杆菌免疫中华鳖所产生的体液免疫应答有所不同。７～９月，抗体效价较高，免疫后第２０ｄ，效价为１∶８，免疫后第３０ｄ，效价为１∶６４，第４０ｄ为１∶１２８；而在冬春季免疫中华鳖后，均未检测到特异性抗体。对其进行２次免疫后，也未见抗体水平升高。还探索了纯化中华鳖血清抗体的条件和方法，并获得高纯度抗体，它与人ＩｇＭ无免疫同源性。

The soft-shelled turtle has different humoral immune response to Aeromonas hydrophilla in different seasons.The titers of antibodies are higher during July to Sep..The titers are 1∶8,1∶64 and 1∶128 on day 20,30,40 after immunization respectively, but no special antibody is detected in the soft-shelled turtle immuned with Aeromonas hydrophilla in spring and winter.After second immunization the titer isn't higher. Some purificating methods and conditions of serum antibody of the soft-shelled turtle are also researched.A kind of pure antibody has been isolated,which has no immune homology with human IgM.