DMSO对泥鳅胚胎脱水量及存活率的影响

Effect of dimethylsulfoxide on dehydrated level and survival rate of loach embryo

本文通过高效液相色谱技术（ＨＰＬＣ），测定了ＤＭＳＯ渗入泥鳅胚胎的过程中单位时间里胚胎内ＤＭＳＯ的含量变化。结果表明，该过程可分为快速进入和慢速进入２个阶段，二者间的转折点出现时间越早，胚胎的存活率就越低。计算了胚胎相应的脱水量，当脱水量达到２５％时，胚胎的存活率为７０％～８０％。尾芽期胚胎对ＤＭＳＯ的耐受力最强，而心跳期胚胎的耐受力最差。

This experiment measured the changes of DMSO contents in the loach embryo cells during its permeating process with HPLC technique.The process divids into 2 courses,i.e.rapid and slow permeation.The turning time of the 2 courses has a close relationship with DMSO concentrations.The earlier the turning time emerges,the lower the survival rate of the embryos is.The dehydrated level of the embyo was calculated. The survival rate is 70%～80% while the dehydrated rate of the embryos 25%.The durability at tail-bud stage was stronger than that at heartbeat stage.