初乳对新生仔猪血液蛋白质影响的研究

STUDY OF COLOSTRUM ON BLOOD PROTEINS OF NEW BORN PIGLETS

将２４头太湖猪新生杂种仔猪（二花×大白），分为４组，即在出生后隔离并分别人工哺喂葡萄糖盐水组（ＧＳ组，ｎ＝４），牛初乳组（ＢＣ组，ｎ＝５），牛乳（ＢＭ组，ｎ＝６）４８小时组和完全自然哺乳的猪初乳组（ＰＣ组，ｎ＝９）。酶联免疫分析法显示，牛ＩｇＧ在ＢＣ和ＢＭ组向仔猪血液中的转移分别在２４和１６小时左右达到峰值，为２８．１９±１５．４６ｍｇ／ｍｌ和１２．８９±１．７８ｍｇ／ｍｌ，以后逐渐下降。４８小时后回圈自然哺乳，第７天两组实验仔猪血中都仍有５ｍｇ／ｍｌ左右牛ＩｇＧ的残留。ＰＣ和ＧＳ组没有检测到牛ＩｇＧ免疫反应物。新生仔猪血清蛋白质浓度初生时平均为３１．８±１．８６ｍｇ／ｍｌ，ＰＣ组在出生后迅速增加，２４小时达８１．７８±８．６ｍｇ／ｍｌ，此后略有下降，４８小时为５６．６７±１１．３６ｍｇ／ｍｌ，第７天为８７．６７±３．８９ｍｇ／ｍｌ。ＢＣ和ＢＭ组与ＰＣ组有相似的变化；而ＧＳ组在４８小时内一直下降，４９小时为６．２５±２．２８ｍｇ／ｍｌ；自然哺乳后，第７天上升为４７．７５±２．６８ｍｇ／ｍｌ。但ＢＭ、ＧＳ组在２４小时、４８小时和第７天血清总蛋白浓度显著低于ＰＣ组（Ｐ＜０．０１）。血清ＳＤＳ－ＰＡＧＥ电泳显示，?

new born Taihu crossed piglets were devided into 4 groups and seperately fed glucose saline(GS,n=4),bovine colostrum(BC,n=5),and bovine milk(BM,n=6)within 48 hours after birth,then back to the lactating sow suckling freely,the piglets in the last group naturally suckled all the time(PC,n=9).Results of ELISA showed that the transfer of bIgG to piglet's serum reached a climax at 24h for BC(28.19±15.64mg/ml) and 16h for BM(12.89±1.78mg/ml)respectively,then declined.About 5mg/ml bIgG in serum of both BC and BM piglets were remained on day 7.The rate of piglets diarrhea in PC and BC were lower than that of piglets in BM and GS.The concentrations of serum proteins of new born piglets were at average of 31.8±1.86mg/ml.Serum protein of PC increased rapidly and reached to 81.78±8.6mg/ml at 24h,then slowly declined to 56.67±11.37mg/ml at 48h,and increased again to 87.67±3.89mg/ml on day 7.The serum protein concentrations in BC and BM changed in the similar way to that in PC.Serum protein of GS kept decreased within 48h(6.25±2.28mg/ml at 48 h),when back to natural feeding,it raised to 47.75±2.68mg/ml on day 7.The serum proteins in BM and GS were significantly lower than that in PC(P<0.01)group at 24h,48h and on day 7.The band of serum albumin on SDS-PAGE gel showed SA in PC,BC and BM groups,constantly increased within 48h,but it declined after first 24h for GS,and some aggregations of serum proteins could be seen around 150kd on GS gel.There were no indications that bovine caseins were intactly absorbed into serum of piglets in BC and BM groups.