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体外受精-胚胎移植过程中未成熟卵体外培养的研究 被引量:1

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摘要 目的研究来源于超排卵周期中的未成熟卵进行体外成熟培养(IVM)的成熟、受精及胚胎发育能力,探讨适宜的未成熟卵体外培养条件,探索IVM技术的临床应用。方法选取68名体外受精/卵胞浆内单精子显微注射-胚胎移植(IVF/ICSI-ET)患者为研究对象,比较采用基本细胞培养基与改良培养基条件下,MI和GV期不成熟卵的体外成熟情况;并比较体内成熟卵和体外成熟卵进行ICSI后的受精、卵裂和优质胚胎形成情况。结果采用常规培养基培养条件下,GV期未成熟卵培养失败,MI期卵成熟率为明显低于改良培养基条件下未成熟卵成熟率;改良培养基条件下GV期与MI期卵成熟率无明显差异(P>0.05);体外培养中69.23%的MI期卵和76.74%的GV期卵均在24h内达到成熟,其24h和48h的成熟率无明显差异(P>0.05);体外成熟卵与体内成熟卵相比较,受精率、卵裂率均无明显差异(P>0.05),优质胚胎形成率较低,差异有显著性(P<0.05)。结论常规超排卵周期中的未成熟卵在改良培养基中能够发育成熟;未成熟卵在拆除卵丘细胞后继续体外发育成熟,具有与体内成熟卵相似的ICSI受精、卵裂能力;虽然优质胚胎的形成率低于体内成熟卵,但增加了胚胎数目,可增加移植胚胎数和冷冻胚胎数量,是一种有效的辅助生殖措施。
出处 《中国优生与遗传杂志》 2010年第1期100-102,共3页 Chinese Journal of Birth Health & Heredity
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同被引文献10

  • 1高敏芝,汪玉宝,顾敦瑜,吴晓云,李忠妹.多囊卵巢患者行未成熟卵母细胞体外成熟、受精和胚胎发育观察[J].生殖与避孕,2005,25(2):89-95. 被引量:9
  • 2徐艳文.卵裂期胚胎的评分[M]//庄广伦.现代辅助生育技术.北京:人民卫生出版社,2005:240-241.
  • 3Yang SH, Son WY, Yoon SH, et al. Correlation between in vitormaturation and expression of LH receptor in cumu- lus cells of the oocytes collected from PCOS patients in HCG-primed IVM cycles [ J ]. Hum Rep rod,2005 (8 : 2097 - 2103.
  • 4Ben-Ami I, Komsky A, Bern O, et al. In vitro maturation of human germinal vesicle-stage oocytes: role of epidermal growth factor-like growth factors in the culture medium [J]. Hum Reprod,2011(l) :76 -81.
  • 5Won H, Park E, Lee D, et al. Comparison of basic medi- um for the in vitro maturation for human immature oocytes [ J]. Fertil Steril,2005 ( Suppl 1 ) :53.
  • 6Hong JY, Yong HY, Ke BC, et al. Effects of amino acids on maturation, fertilization and embryo deveh) pment of pig follicular oocytes in two IVM medial[ J ]Iheriogenology, 2004(8) :1473 - 1482.
  • 7Tao Y, Zhou B, Xia G, etal. ExposuretoL-ascorbic acid oralpha-toeopherol facilitates the development of porcine denuded oocytes from metaphase 1 to metaphase 11 and prevents cumulus cells from fragmentation [ J ]. Reprod Do- mest Anim,2004( 1 ) :52 -57.
  • 8金美善;黄国宁,等.体外授精一胚胎移植实验室技术[M].北京:人民卫生出版社,2012:191-195.
  • 9冯婷,王守林,刘嘉茵,钱晓乔,蔡令波.两种未成熟卵母细胞体外成熟培养液的运用效果比较[J].南京医科大学学报(自然科学版),2010,30(1):121-127. 被引量:5
  • 10刘晓音,金炜,薛松果,曹少锋,傅永伦,彭秋平,吕祁峰,匡延平.一种简易的促排卵周期挽救性卵子体外成熟培养技术[J].中山大学学报(医学科学版),2010,31(2):293-297. 被引量:3

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