正常和活力低下的人精子膜脂类过氧化反应或环核苷酸与运动性的关系(英文)

RELATIONSHIPS BETWEEN LIPID PEROXIDATION OR CYCLIC NUCLEOTIDES AND MOTILITY IN HUMAN ASTHENOZOOSPERM AND NORMOSPERM

本文分别采用过膜游动法、硫代巴比妥酸比色法和放射免疫分析法测定了正常人和精子活力低下的不育症患者精子膜的脂类过氧化反应(LP)、环一磷酸腺苷(cAMP)和环一磷酸鸟苷(cGMP)水平,研究了膜LP、细胞内cAMP和cGMP与运动性之间的关系。结果表明,无论正常还是活力低下的精子,膜LP和运动性之间均存在明显负相关(r=-0.76,P<0.001和r=-0.68,P<0.001);精子内两种环核苷酸与运动性之间则均存在明显正相关(cAMP:r=0.64,P<0.01和r=0.59,P<0.01;cGMP:r=0.60,P<0.01和r=0.55;P<0.05)。其中膜LP与运动性之间的相关性最高。这些结果提示,膜LP是造成精子运动性降低的主要原因之一;精子内两种环核苷酸也可能影响其运动性。

The relationships between sperm lipid per-oxidation (LP) or cyclic nucleotides and sperm motility in normospermic and asthenozoospermic specimens were analyzed. Sperm motility was measured by the transmembrane migration method; LP was measured by thiobarbituric acid (TBA) method and the intracellular cAMP and cGMP contents was measured by radioim-munoassay in 20 fertile and 20 asthenozoospermic infertile human semen specimens. Results showed that in both fertile and infertile individual , there was a close negative correlation between sperm LP formation and motility (r=-0.76; P <0. 001 and r =-0. 68; P<0. 001); there were significant positive correlations between intracellular cAMP (r = 0. 64; P<0. 01 and r = 0. 59; P <0. 01) or cGMP (r = 0. 60; P<0. 01 and r = 0. 55;P<0. 05) and sperm motility; and the correlation between LP and motility was the closest. These results suggest a causative role for LP in the aetiology of male infertility due to defective sperm motility, and confirmed that intra-梒ellular cyclic nucleotides likely also have influences on sperm motility.