rhlL-6影响巨噬细胞内钙离子浓度和空间分布

EFFECT OF rhIL-6 ON MACROPHAGE INTRACELLULAR CALCIUMCONCENTRATION AND DISTRIBUTION

使用重组人白细胞介素６（ｒｈＩＬ－６）刺激小鼠腹腔巨噬细胞，应用荧光分光光度计和激光扫描共焦显微镜检测胞浆内游离钙离子的时空分布。静息状态巨噬细胞Ｃａ２＋浓度１０－８｀～１０－７ｍｏｏ／Ｌ，加入１００ｕ／ｍｌｒｈＩＬ－６后，未见作用。从２００ｕ／ｍｌ至４００ｕ／ｍｌ不同浓度的ｒｈＩＬ－６则使细胞内游离Ｃａ２＋浓度呈尖峰状迅速升高３００％左右，然后急剧下降至静息值。且各个浓度对Ｃａ２＋的升高幅度及峰形基本一致，但随浓度的升高，Ｃａ２＋浓度达到高峰所需的时间逐渐缩短。这说明，胞外ＩＬ－６浓度存在着一阈值，只有达到或超过这一阈值。

In this study, the effects of rhIL-6 on intracellular calcium of mice peritoneal macrophages were investigated by Fluorescence Spectrophotometer and Laser Scanning Confocal Microscope(LSCM). The result shows that the basal i in resting macrophages is about 10-8～10-7mol/L. When rhIL-6 of 200～400u/ml was added, a rapid increase of i was obtained with an average increase about 300% of the basal value, whereas with rhIL-6 of 100u/ml no effect was seen. It shows that rhIL-6 exposure above 200u/ml induce a sharp increase in i following different time-delay. This time-delay was dose-dependent with the shortest at 400u/ml. These results suggested that a minimal concentration of rhIL-6 is required to initiate the rapid increase of i.