摘要
γ－微管蛋白是一种新发现的与微管形成有关的蛋白质[1]。利用PCR技术从一种下毛类纤毛虫─—八肋游仆虫大核DNA中扩增出γ－微管蛋白基因并对其核昔酸序列进行了分析。用抗γ－微管蛋白的多克隆抗体标记八肋游仆虫发现：γ－微管蛋白存在于八肋游仆虫的纤毛、棘毛的基部─—即基体的部位。另外，在有些分裂间期及有丝分裂期的小核内以及部分大核内也发现有γ－微管蛋白的存在。
γ-tubulin is a newly discovered protein , which is considered to be essentialfor microtubule nucleation. With the help of the polymerase chain reaction(PCR) weamplified and sequenced the γ-tubulin gene of Euplotes octocarinatus, a hypotrichousciliate. Polyclonal antibody to localize mis protein in E. octocarinatus and found, thatγ-tubulin is present in the basal bodie of dordal cilia,the basal bodies of cirri and the basal bodies of membranelles. In addition ,it was found in the micronucleus and andin the macronucleus.
出处
《水生生物学报》
CAS
CSCD
北大核心
1998年第4期325-329,共5页
Acta Hydrobiologica Sinica
基金
国家自然科学基金
