褪黑素对小鼠免疫性肝损伤的保护作用

Protective effects of melatonin on immunological liver injury in mice

目的观察褪黑素（MT）对小鼠免疫性肝损伤的影响。方法序贯注射短小棒状杆菌和脂多糖诱导小鼠免疫性肝损伤模型;在造模不同时间注射不同剂量MT;检测血浆转氨酶、肝脏雨二醛（MDA）和谷胱甘肽过氧化物酶（GSH-px）以及脾淋巴细胞增殖状况。结果造模期间用MT（0.1～10.0mg·kg-1）能显著降低血浆转氨酶和肝脏MDA水平（P＜0.05～0.01），使肝脏GSH-px活性部分恢复（P＜0.05），并能选择性抑制刀豆蛋白A诱导的脾淋巴细胞增殖。但造模后使用MT对免疫性肝损伤无明显影响。结论MT保护免疫性肝损伤作用与其抗氧化和免疫调节作用有关。

AIM To investigate the effect of melatonin (MT) on immunological liver injury in mice. METHODS An immunological liver injury model was established by injection of lipopolysaccharide into corynebacterium parvum-primed mice, while different dosage of MT was given at the different time intervals. The changes of plasma transaminase levels, the content of liver MDA, liver GSH-px activity and the proliferation of splenocytes were comparatively observed to evaluate the effects of MT on immunological liver injury. RESULTS When MT (0. 1～ 10. 0 mg·kg-1) was given during the evolution of the animal model, plasma transaminase levels were reduced (P＜0. 05 ～0. 01 ), the content of liver MDA was decreased (P ＜ 0.01) and liver GSH-px activity was partially restored, while Con A-induced proliferation of splenocytes was significantly suppressed (P＜0. 01). But the inhibitory effect of MT of immunological liver injury was not showed when it was given after the induction of the animal model. CONCLUSIONS MT has protective effect on immunological liver injury in mice, which may be due to its antioxidative action and immunological regulation.