HPD300型大孔吸附树脂分离纯化白芍总苷的工艺研究

Purification of total glycosides of paeony by HPD300 macroporous resin

目的:优化HPD300型大孔吸附树脂分离纯化白芍总苷的工艺条件。方法:考察上柱药液的pH值、药液的浓度、柱径/柱高的比例、洗脱乙醇浓度、洗脱溶剂用量、上样流速考察、最大上样量及洗脱流速,确定纯化工艺条件。结果:HPD300型大孔吸附树脂分离纯化白芍总苷的工艺条件为上样浓度0.1g生药/mL,流速4BV.h-1,树脂柱径/高比为1∶11,以5倍柱体积水洗脱,继以7倍柱体积50%乙醇洗脱。结论:确定工艺条件下,纯化白芍总苷效果良好,芍药苷的纯度可达50%以上,白芍总苷纯度可达80%以上。

OBJECTIVE To optimize the best condition of purification of total glycosides of Paeany（TGP） by HPD300 macroporous resin. METHODS The following factors were studied： the pH of the liquid, the concentration of liquid, column drive/column high proportion, elution ethanol concentration, eluting solvent amount, sample flow rate, the largest sample volume and elution velocity, from which we got the process conditions by macroporous resin to separate and purify TGP. RESULTS The HPD3011 macroporous resin was used to separate TGP in the following condition： the sample concentration was 0.1g·mL^-1 , 4 BV·h^-1 through macroporous resin column, （resin column Drive/high was 1 ： 11 ）, used five times volume of water to wash first, following by seven times volume of 50% ethanol to wash and the elution velocity was 4 BV·h^-1. CONCLUSION TGP can he successfully purified in the above condition, more than 80% of the TGP content and 50% paeoniflorin content are available.