H-2~b和H-2~d单体型小鼠MHCI类分子结合的HFRSV蛋白抗原表位的预测

Prediction of epitopes on HFRSV nucleocapsid protein and M polyprotein precursor restricted by H 2 d and H 2 b class Ⅰ molecules

本文通过对Ｈ－２ｂ和Ｈ－２ｄ单体型小鼠主要组织相容性抗原Ｉ类分子结合抗原肽的分析，建立了一个计算机预测模型，并对肾综合征出血热病毒核蛋白（ＮＰ）及囊膜糖蛋白Ｇ１和Ｇ２可与Ｂａｌｂ／ｃ小鼠（Ｈ－２ｄ）和Ｃ５７ＢＬ／６（Ｈ－２ｂ）小鼠ＭＨＣＩ类分子相结合的抗原表位进行了分析，预测出可分别与Ｈ－２Ｋｂ，Ｈ－２Ｄｂ，Ｈ－２Ｋｄ，Ｈ－２Ｄｄ和Ｈ－２Ｌｄ结合的核蛋白表位，依此各有７，１４，４，０和８个；囊膜糖蛋白Ｇ１＋Ｇ２的表位，依次有１４，１９，２７，２和４４个。

We analyzed the common motif restricted respectively by mouse H 2 d and H 2 b class I molecules, and a computer program that searches epitopes deduced from the H 2 d and H 2 b specific peptide binding motifs was designed. Using this program, we got the possible binding epitopes of HFRSV nucleocapsid protein and M polyprotein precursor containing glycoprotein G1 and G2, which completely coordinated with the given specific peptide binding motifs.There were 7, 14,4, 0 and 8 epitopes on HFRSV nucleocapsid protein which could be restricted respectively by H 2K b, H 2D b, H 2K d, H 2D d and H 2L d , and for HFRSV M polyprotein precursor, there were 14, 19, 27, 2 and 44 epitopes respectively.