胃癌组织bcl-2蛋白表达和微卫星不稳定性研究及其临床意义

Clinical Significance of Expression of bcl 2 Protein and Microsatellite Instability in Gastric Cancer

目的：研究ｂｃｌ－２蛋白过度表达和染色体１８ｑ微卫星不稳定性（ＭＳＩ）在胃癌发生及发展过程中的作用。方法：采用ＰＣＲ为基础的方法和免疫组织化学技术检测５０例手术切除胃癌标本的微卫星不稳定性及ｂｃｌ－２蛋白表达。结果：胃癌ｂｃｌ－２蛋白表达阳性率为６０％（３０／５０）；ＭＳＩ检出率为３０％（１５／５０）；中～高分化腺癌ＭＳＩ发生率（５３．３％，８／１５）显著高于低分化腺癌（１６．２％，５／３１）（Ｐ＜０．０５）。ｂｃｌ－２蛋白表达与ＭＳＩ无显著相关。结论：ｂｃｌ－２蛋白过度表达及ＭＳＩ（１８ｑ）在胃癌的发生中均发挥重要作用。

In order to investigate the role of bcl 2 protein overexpression and microsatellite (MSI) instability in the development of gastric cancer (GC),bcl 2 protein and MSI were detected by using the immunohistochemical technique and PCR bcl 2 protein was determined in 30/50 of GC, and MSI in 15/50 of GC. The frequency of MSI (53.3%, 8/15) in well differentiated group was significantly higher than that in poorly differentiated group (16.2%,5/13) ( P <0.05). No significant correlation was found between MSI and bcl 2 protein expression. The results suggest that the overexpression of bcl 2 protein and MSI (18q) may play an important role in the carcinogenesis of GC, however, the carcinogenic mechanism may be different between the two.