多重肿瘤抑制基因p16在喉癌及边缘组织和声带息肉中表达的研究

Study on Expression of Multiple Tumor Suppressor Gene p16 in Laryngeal Cancer and Its Adjacent Tissue and Vocal Cord Polyp

目的：为了探讨ｐ１６基因在喉癌及边缘和声带息肉组织中的表达情况，研究其与喉癌临床间的关系。方法：采用免疫组化ＳＡＢＣ方法对３６例原发喉癌及边缘１．０ｃｍ组织和２０例声带息肉组织中ｐ１６蛋白检测。结果：喉癌组１０例阳性率２７．８％，边缘组２０例阳性率５５．６％，息肉组８例阳性率４０％。喉癌组与边缘组阳性率之间差异显著Ｐ＜０．０５，喉癌组中，阳性率明显高于强阳性率，两者之间差异明显，Ｐ＜０．０５。高分化组阳性率明显高于低分化组阳性率，Ｐ＜０．０５。ｐ１６蛋白的表达在喉癌的临床分期，肿瘤发生部位及是否有淋巴结转移间差异不明显。

The expression of p16 gene protein was determined immunohistochemically in 36 cases of primary laryngeal cancer and their adjacent tissue (1.0cm to the tumor) and 20 cases of vocal cord polyp. The results showed that the positive rates were 27.8% in cancer, 55.6% in adjacent tissue groups and 40% in vocal cord polyps. There was significant statistic difference of positive rate between the cancer and adjacent tissue groups ( P <0.05). Of the cancer group, the case marked positive significantly outnumbered those marked strongly positive ( P <0.05). The positive rate of the well differentiated cases was significantly higher than that of the poorly differentiated ( P <0 .05).The expression of p16 did not show apparent association with the clinical staging or lymph node metastasis. The results suggested that p16 plays an important role in inhibiting proliferation and differentiation of laryngeal cancer cells.