加味菖蒲郁金汤对抑郁大鼠模型行为学的影响

Effect of ChangPu Yujin Decoction on the Behaviors in Rats with Depression Model

目的:观察加味菖蒲郁金汤对抑郁大鼠模型行为学指标的影响。方法:经Open-Field法[1-3]评分测定评分相近的48只SD普通级大鼠,体质量为(200±20)g,雌雄各半,随机分为空白对照组(A)、模型组(B)、加味菖蒲郁金汤组(C)、氟西汀组(D)共4组,每组各12只。除对照组外,其他各组采用孤养和长期不可预见的慢性应激两种方法复制大鼠抑郁症模型[1]。以Open-Field法和糖水消耗实验对照研究慢性应激诱导的抑郁症模型大鼠行为学指标。结果:①与A组比较,各模型组大鼠在模型复制第21天Open-Field评分降低,糖水消耗量减少;②与B组比较,治疗组大鼠经治疗后Open-Field评分升高,糖水消耗量增加。结论:加味菖蒲郁金汤可改善孤养和长期不可预见的中等强度应激诱导的抑郁大鼠模型的抑郁行为。

Objective： To observe the effect of ChangPu Yujin decoction on the index of the behaviors in rats with depression model. Methods： 48 SD rats with similar score method were selected according to Open-Field.The rats of body mass （200±20） g and male and female in half were randomly divided into four groups, control group（A）, model group （B）, ChangPu Yujin decoction group（C） and fluoxetine group（D）, each group 12 animals. In addition to the control group, two ways of support and long-term use of solitary unpredictable chronic stress in other groups were adopted to copy the rat model of depression. The indexes of the behaviors of chronic stress-induced depression model were measured by the method of Open-Field and sugar consumption experiment. Results： （1） Compared with the A group, Open-Field score and sugar water consumption of the model group reduced in 21 days to duplicate model. （2）Compared with the B group, in the treatment group after treatment, Open-Field score risen and sugar water consumption increased. Conclusion： ChangPu Yujin decoction can change depression behavior of rats with depression model of the solitary and long-term unforeseeable moderate intensity.