SLE病人外周血Bcl-2/JH基因重排检测的意义

Bcl 2 REARRANGEMENTS IN PERIPHERAL BLOOD MONONUCLEAR CELLS OF PATIENTS WITH SYSTEMIC LUPUS ERYTHEMATOSUS

应用聚合酶链反应（ＰＣＲ）方法检测３１例ＳＬＥ病人外周血单个核细胞（ＰＢＭＣ）Ｂｃｌ－２／ＪＨ基因重排现象和流式细胞仪间接双标记法分析其Ｔ（ＣＤ３）、Ｂ（ＣＤ１９）细胞Ｂｃｌ－２蛋白的表达。结果显示，ＳＬＥ病人Ｔ细胞Ｂｃｌ－２蛋白表达明显高于正常人（４２．９５％±２８．４７％对比９．９４％±４．９６％，Ｐ＝０．０００４），尤其以活动期ＳＬＥ病人为明显，而Ｂ细胞Ｂｃｌ－２蛋白表达与正常人之间并无统计学差异（７９．２１％±１０．６９％对比８１．９６％±６．９７％；Ｐ＝０．４６０２）。７例ＳＬＥ病人具有典型的Ｂｃｌ－２／ＪＨ基因重排（占２２．５８％），且均为ＳＬＥ活动期病人，其Ｔ细胞Ｂｃｌ－２蛋白表达明显高于无基因重排的ＳＬＥ病人，其Ｂ细胞Ｂｃｌ－２表达并无差异（Ｐ＞０．３９０５）。说明Ｂｃｌ－２／ＪＨ基因重排现象可见于ＳＬＥ，并与Ｔ细胞Ｂｃｌ－２蛋白高表达有关，表明细胞凋亡抑制基因Ｂｃｌ－２在ＳＬＥ发病机制中具有重要作用。

Bcl 2/JH rearrangements in peripheral blood mononuclear cells(PBMC) were tested by polymerase chain reaction(PCR) analysis in 31 SLE patients,and levels of Bcl 2 in T,B cells were examined by two color cytofluorography.Compared with normal controls,a proportion of T cells expressed Bcl 2 protein increased significantly in SLE patients,specially in active patients,Bcl 2 rearrangements in PBMC were founded in 7 SLE patients,their Bcl 2 protein proportions in T cell,but not B cells were expressed significantly more than SLE patients without Bcl 2 rearrangements.In conclusion,Bcl 2 rearrangements are not restricted to follicular lymphoma cells,In SLE patients,this gene rearrangements can be detected by PCR,and this rearrangements are correlated with Bcl 2 protein proportions of T cells.Bcl 2 gene might play a role in the pathogenesis of SLE in some cases.