烧伤病人TNF、IL-6的变化与氧自由基关系的研究

THE CORRELATION STUDY BETWEEN THE TUMOR NECROSIS FACTOR(TNF),INTERLEUKIN 6(IL 6) AND THE OXYGEN FREE RADICAL (OFR) IN BURNED PATIENTS

研究烧伤后ＴＮＦ、ＩＬ－６的变化与氧自由基的关系。采用酶标法检测血清ＴＮＦ和ＩＬ－６，改良八木国夫法检测血清ＭＤＡ，动态观察了烧伤病人伤后１０ｄ内血清ＴＮＦ、ＩＬ－６和ＭＤＡ的变化以及抗氧化剂对血清ＴＮＦ和ＩＬ－６的影响。结果显示烧伤病人血清ＴＮＦ、ＩＬ－６和ＭＤＡ均显著增高（Ｐ＜０．０１）；ＴＮＦ与ＭＤＡ的变化呈正相关（ｒ＝０．４５，Ｐ＜０．０５）；抗氧化剂可降低血清ＴＮＦ（Ｐ＜０．０５）和ＩＬ－６（Ｐ＜０．０１）。说明烧伤后ＴＮＦ、ＩＬ－６产生增多与氧自由基有关。

To study the correlation between the TNF,IL 6 and OFR after burn.Measuring the serum TNF,IL 6 with enzyme linked immunosorbend assay(ELISA) and malonyldialdehyde(MDA) with thiobarbituric acid spectrophotometer assay,we monitored the changes of the serum TNF,IL 6 and MDA following ten days and after treating with antioxidants in burned patients.The results showed that the serum TNF,IL 6 and MDA were elevated significantly in burned patients(P<0 01);the changes between the serum TNF and MDA was pasitive correlation(r=0 45,P<0 05);antioxidants can decrease the serum TNF and IL 6 in burned patients (P<0 05 and P<0 01).Conclousion:OFR was one of causes of the serum TNF,IL 6 increase following burn.