IL-6基因转染的骨髓基质细胞系对骨髓移植小鼠造血功能恢复的促进作用

Effect of the IL-6 transfected bone marrow stromal cells on the recovery of hematopoietic functions in bone marrow transplanted mice

目的:探讨白细胞介素6(IL-6)基因转染的骨位基质细胞系QXMSC, IL6对骨髓移植后造血功能的重建作用。方法:将骨髓造血细胞和骨髓基质细胞系一起经尾静脉注射给同系小鼠,建立骨髓移植(BMT)模型。小鼠的造血功能用脾结节(CFU-S)、粒-单系祖细胞(CFU-GM)、红系祖细胞(CFU-E、BFU-E)测定及外周血各项血液学指标来确定。结果:QXMSC_1 IL-6转基因骨髓基质细胞可明显增强BMT小鼠形成的CFU-S、CFU-GM、CFU-E和BFU-E数,促进外周血象的恢复。结论:QXMSC_1 IL-6细胞可明显促进小鼠骨髓移植后早期造血功能重建。

Objective:To study the effect of the IL-6 transfected bone marrow stromal cell, QXMSC_1 IL-6, on the hematopoietic reconstitution in bone marrow transplanted mice. Methods: Bone marrow transplan- tation model was established by infusing hematopoietic cells combined with QXMSC_1 IL-6 into syngeneic recipi- ent mice through the tail vein. CFU-S, CFU-GM, CFU-E, BFU-E, and peripheral blood pictures were as- sayed. Results: QXMSC_1 IL-6 could increase the CFU-S, CFU-GM, CFU-E and BFU-E yields in BMT mice, and accelerate the recovery of peripheral blood cells. Conclusion: QXMSC_1 IL-6 can improve the hematopoietic reconstitution in bone marrow transplanted mice.