原发性肝癌患者血清中庚型肝炎病毒核酸的检测分析

Detection of HGV RNA in Sera of Prirary Liver Cancer Patinets

为了解庚型肝炎病毒（HGV）在原发性肝癌患者中的感染状况，本文对145例原发性肝癌患者血清进行了分析。应用逆转录多聚酶链反应（RT—PCR）技术扩增庚型肝炎病毒基因，采用蛋白酶K裂解法提取血清中HGV—RNA，逆转录为CDNA后进行巢式扩增，获得238bp的特异性片段。用此方法对145例原发性肝癌（HCC）患者血清标本进行检测，结果发现21例HGV－RNA阳性（14．5％），均为男性患者。

Investigation of the hepatitis G virus(HGV) infection status among hepatocellular carcinoma (HCC) patients. Proteinase K digestion method was used to extract HGV RNA in sera. RT-nested PCR technique was applied to amplify the 238bp HGV genome fragment. Serum specimens of 145 cases of HCC have been tested. HGV RNA was positive in 21 (14. 5%) male patients out of 145 cases of HCC. The result sugested that HGV infection might be related with HCC.