平原和模拟高原条件下梭曼中毒对AChE活力影响的比较

EFFECT OF SOMAN INTOXICATION AT HIGH ALTITUDE ON ACETYL CHOLINE ESTERASE ACTIVITY COMPARED WITH THAT AT PLAIN IN RATS AND DOGS

采用DTNB显色法 ,研究平原和模拟 40 0 0m高原缺氧条件下梭曼对犬、大鼠组织及全血AChE活力的影响。结果显示 :(1)缺氧可抑制AChE活性。(2 )模拟高原条件下梭曼对两种动物AChE的抑制和平原条件下一样 ,均为持续性抑制 ,中毒后 2hAChE仍无明显回升。 (3)缺氧复合梭曼中毒对犬全血AChE的抑制速度和抑制程度都较平原中毒组有增高的趋势。而对于大鼠组织来说 ,其AChE的抑制速度明显快于平原中毒组 ,抑制程度也偏重 ,且存在一定的剂量效应关系。(4)缺氧复合梭曼中毒对大鼠全血AChE的抑制峰提前出现 ,但抑制程度相对轻些。以上结果表明在缺氧条件下血液中AChE的活性变化与梭曼的毒性并不呈平行关系 ,而脑组织中AChE的变化一定程度上可反映梭曼的毒性程度。

DTNB method was used in these experiments to investigate the effect of soman intoxication under high altitude hypoxia on blood or brain acetyl choline esterase(AChE)activities compared with that at plain in dogs and rats. The results showed that the activity of AChE can be inhibited by hypoxia. The inhibition induced by soman to the activity of AChE in rats and dogs at high altitude, consistent with that at plain, was continuous. Two hours after soman injection, the activity of AChE was still significantly lower than that of control. The speed and degree of AChE inhibition in rat brain induced by soman and hypoxia both are higher than that at plain.There was a dose-dependent effect on brain AChE inhibition in rats. There only was a higher speed inhibition of AChE in rat whole blood at high altitude, but the degree of AChE inhibition was little smaller than that at plain. These indicated that AChE activity in brain, but not blood, could express the soman intoxication under hypoxia.