高效液相色谱法测定烟草中的果糖、葡萄糖和蔗糖

Determination of Fructose,Glucose and Sucrose in Tobacco with High Performance Liquid Chromatography

以72%（体积比）乙腈/28%水预混合溶液作流动相,High-Performance Carbohydrate卡套柱作色谱柱,等梯度洗脱,采用带示差折光检测器的高效液相色谱仪测定了烟草中的果糖、葡萄糖和蔗糖含量。结果表明：方法的日内精密度和日间精密度的范围分别为0.46%~1.54%和0.96%~4.74%,回收率97.0%~102.3%,果糖、葡萄糖和蔗糖的检出限（LOD）分别为95,107和105μg/mL（S/N=3∶1）,可以满足烟草中果糖、葡萄糖和蔗糖含量的日常检测要求。

The fructose,glucose and sucrose in tobacco were determined by high performance liquid chromatography with a High-Performance Carbohydrate cartridge column and a differential refractive index detector at isocratic mode using a premixed solution of 72% acetonitrile-28% water（v/v） as an eluent.The results showed that intra-day and inter-day precisions were in the range of 0.46%-1.54% and 0.96%-4.74%,respectively.The recovery was between 97.0%-102.3%.The limits of detection of fructose,glucose and sucrose were 95,107 and 105 μg/mL,respectively.This method meets the requirements for the routine analysis of these sugars in tobacco.