兔脑创伤后早期血清β淀粉样前体蛋白动态变化的实验研究

The Dynamical Changes of Serum β-APP in Early Stage of Rabbit Traumatic Brain Injury

目的探讨兔脑创伤后血清β淀粉样前体蛋白(β-APP)含量与损伤程度及其时间动态变化相关性。方法将24只大白兔随机分为对照组和轻、中、重度脑损伤组,各组于不同时间点(0.5h、2h、6h、24h)采集标本,检测血清β-APP的含量。结果采用完全随机设计多因素重复测量方差分析和组间两两比较的LSD检验进行统计学分析。结果脑损伤后血清β-APP均升高。中、重度损伤组各时间点血清β-APP含量较对照组和轻度损伤组升高(P<0.05),重度损伤组血清β-APP含量各时间点均高于同时间点的中度损伤组,但差异无统计学意义(P>0.05);轻度损伤组血清β-APP含量与对照组相比在统计学上无显著性差异(P>0.05)。结论脑损伤后血清β-APP含量升高,且损伤越重血清β-APP含量越高。血清β-APP含量可作为早期判断颅脑损伤程度指标之一。

Objective To explore the relationship of serum levels of β-amyloid precursor protein （β-APP）with degree of traumatic brain injury （TBI）and the traumatic time. Methods Sprague-Dawley （SD）rats were randomly divided into normal control and injury group. The rats in injury groups suffered from TBI after free-falling percussion with different pressure（wild-injury,moderate-injury and severe-injury group）. Then serum was collected at 0.5 h,2 h,6 h,and 24 h and subject to β-APP detection by ELISA. All data were analyzed statistically with completely randomized design multiple factor repeated measure of variance analysis and least significant difference （LSD）test. Results The serum levels of β-APP were higher after injury. The serum levels of β-APP were significantly higher in moderate-injury or severe-injury group than those in normal group or slight-injury group（P 〈 0.05）. The serum levels of β-APP were higher in severe-injury group than that in moderate-injury group with no statistical difference （P 〉 0.05）. There was no statistical difference in serum β-APP levels between normal control and slight-injury group （P 〉 0.05）. Conclusion The serum level of β-APP is increasingly higher with traumatic brain injury more serious and could be employed as an indicator of TBI degree. It implies that β-APP has the potential as an early diagnosis marker for TBI.