深圳地区HIV-1流行毒株的env基因序列测定和亚型分析

Subtype and sequence analysis of the C2 V3 region of gp120 genes among HIV 1 strains in Shenzhen

目的了解深圳地区ＨＩＶ－１各亚型毒株的流行情况和传播方式。方法用套式－聚合酶链反应（ｎｅｓｔｅｄ－ＰＣＲ）对５份在深圳市发现的ＨＩＶ－１感染者外周血单个核细胞（ＰＢＭＣ）中的ＨＩＶ－１膜蛋白基因进行扩增，并对Ｃ２－Ｖ３及其邻区３５０－４５０个核苷酸序列进行测定和分析。结果５份样品中存在Ｂ和Ｅ两种亚型的ＨＩＶ－１毒株序列，其中Ｂ亚型２份，彼此间的基因离散率为１０．２％；Ｅ亚型３份，彼此间的基因离散率为２．７％。Ｂ亚型株与欧美Ｂ亚型毒株序列十分接近，Ｅ亚型株与泰国Ｅ亚型共享序列非常相似。结论深圳地区ＨＩＶ－１Ｂ亚型为经性途径由境外传入。

Objective To study the prevalence status and transmission patterns of various subtypes of HIV 1 in Shenzhen.Methods HIV 1 env gene was amplified by PCR from uncultured peripheral blood mononuclear cells (PBMCs) obtained from 5 HIV 1 seropositive infectors of Shenzhen in 1996.The C2 V3 region (about 450bp) of them was sequenced.Results There are two HIV 1 subtypes,B and E with 10.2% and 2.7% gene divergence inside each subtype.The 2 subtypes were closely related to the type B in Europe and America.Meanwhile,the 3 subtypes of E strains were most close to the international subtype E.Conclusion This study and epidemiology references showed that the subtype B was transmitted by sex and the subtype E was transmitted by drug users as well as blood donors in Shenzhen.