乙型肝炎病毒核心区基因免疫小鼠细胞免疫应答研究

The immune response in Balb/c mice immunized with hepatitis B virus core DNA based vaccine

目的观察小鼠对ＨＢＶ核心区基因疫苗的免疫应答。方法将经过基因修饰的乙型肝炎病毒核心区基因定向克隆于真核表达载体（ｐｃＤＮＡ３），构建成ＨＢＶ核心区基因疫苗（ｐｃＤＮＡ－ＨＢＶ），给Ｂａｌｂ／ｃ小鼠多点肌肉注射，２周后追加免疫一次，用竞争抑制酶联法及ＭＴＴ法检测小鼠血清抗－ＨＢｃ及脾细胞对ＨＢｃＡｇ的特异性增殖反应。结果免疫接种２周后小鼠血清抗－ＨＢｃ均阳性，持续１２周以上，脾细胞对ＨＢｃＡｇ的刺激指数明显高于对照组（Ｐ＜０．０５）。结论ｐｃＤＮＡ－ＨＢＶ在Ｂａｌｂ／ｃ小鼠体内既可诱导体液免疫应答。

Objective To study immune response in mice against the HBV core DNA based vaccine.Methods The modificated HBc gene with its stem and loopstructure was inserted into the eukaryotic expression vector (pcDNA HBV).The Balb/c mice were immunized by multiple sites intramuscular injection with pcDNA HBV and pcDNA3 respectively.Each mouse was boosted after 2 weeks.The anti HBc Ab was detected by ELISA,meanwhile,the stimulation index of spleencytes to HBcAg were measured by MTT method.Results The anti HBc in mice immunized with pcDNA HBV became and maintained to be positive for at least 12 weeks.The stimulation index of spleencytes to HBcAg were significantly higher than that of mice injected with pcDNA3.Conclusion The investigation demonstrated that pcDNA HBV could generated humoral and cellular immune response in Balb/c mice.