佛山市庚型肝炎病毒检测和部分基因序列分析

Detection of hepatitis G virus infection and sequencing its partial gene in Foshan district

目的了解佛山庚型肝炎病毒（ＨＧＶ）感染状况，分析ＨＧＶ非结构基因（ＮＳ）３区部分核苷酸序列。方法采用逆转录聚合酶链反应检测血清ＨＧＶＲＮＡ，对一例肝炎患者的ＨＧＶ（ＨＧＶＣ－ＦＳ）ＮＳ３区８１８ｂｐ片段作克隆及序列分析。结果８０例非甲－戊型肝炎患者和１０５例静脉吸毒者ＨＧＶＲＮＡ检出率分别为６．３％（５／８０）和２３．８％（２５／１０５），ＨＧＶＣ－ＦＳＮＳ３区片段核苷酸序列与ＨＧＶ－Ｕ４４４０２、Ｕ４５９６６、Ｕ３６３８０及ＨＧＶＣ９６４相同区段同源性为８５．５％、８５．６％、８８．０％、８９．２％。结论佛山存在ＨＧＶ感染，静脉吸毒者感染率较高，ＨＧＶ可能不是非甲－戊型肝炎主要致病因素。ＨＧＶＣ－ＦＳ与ＨＧＶＣ９６４同源性最高。

Objective To investigate the HGV infection in Foshan and analyse partial nucleotide sequence of the putative nonstructural 3 (NS3) region of HGV (HGVC FS) isolated form a patient with post transfusion non A E hepatitis in Foshan.Methods HGV RNA in the sera of 80 patients with non A E hepatitis and 105 intravenous drug abusers (IVDU) was detected by reverse transcription polymerase chain reaction.A 818bp fragment from NS3 region of HGV isolated from the serum of a patient with post transfusion non A E hepatitis was cloned and sequenced.After molecular cloning,it was sequenced by Sanger′s method and compared with those of the HGVU44402 U45966 U36380 reported in America and HGVC964 reported in China.Results The positive rates of HGV RNA in 80 patients with non A E hepatitis and 105 IVDUs were 6.3%(5/80) and 23.8%(25/105) respectively.The nucleotide homology between HGVC FS and HGVU44402,U45966,U36380,HGVC964 were 85.5%,85.6%,88.0% and 89.2% respectively.Conclusion There are hepatitis G virus infection in Foshan district.The infection rate was higher in IVDU.HGV may be not the dominate pathogen of non A E hepatitis.HGVC FS is highest homologous to HGVC964.