高效液相色谱法测定格列喹酮的血药浓度

HPLC Determination of Gliquidone In Human Plasma

建立了格列喹酮血药浓度的测定方法。该法使用ＳｐｈｅｒｉｓｏｒｂＣ１８色谱柱，以乙腈－水（６５３５）为流动相，流速为１．０ｍｌ／ｍｉｎ，检测波长为３１０ｎｍ，灵敏度为０．００１ＡＵＦＳ。线性范围为５０～２０００ｎｇ／ｍｌ，方法回收率为９５％以上，日内、日间误差小于７．０％。

A HPLC method was established to determine the plasma concentration of gliquidone The method utilized a Spherisorb C 18 column, CH 3CN H 2O (65∶35) as mobile phase with the flow rate of 1 0 ml/min and 310 nm as detective wavelength at a sensitivity of 0 001 AUFS Gliquidone was abstracted by 4 ml methylene dichloride from 1 ml plasma, then 3 ml methylene dichloride was transfered to another tube and dried by air at 50℃, the residue was dissolved by 100 μl mobile phase and 20 μl was injected to the column The standard curve showed a linearity in the range of 50￣2000 ng/ml The recovery of the method was over 95% The Intra day and Inter day RSD were less than 7 0%