BMP-7与IL-6对人髓核细胞ColⅡ与TIMP-1基因表达的影响

Interaction of Bone Morphogenetic Protein-7 and Interleukin-6 on Gene Expression of TypeII-collagen and TIMP-1 in Human Nucleus Pulposus Cells in Vitro Cultured

目的探讨不同浓度BMP-7与IL-6作用于体外培养的人髓核细胞对Ⅱ型胶原与TIMP-1基因表达的影响。方法应用逆转录聚合酶链反应(RT-PCR)半定量检测不同浓度BMP-7与IL-6作用下体外培养的人髓核细胞中TIMP-1和Ⅱ型胶原基因的表达量。结果IL-6可以下调Ⅱ型胶原基因和TIMP-1基因表达;低浓度的BMP-7可以抵消相同浓度IL-6对髓核细胞的保护作用,高浓度的BMP-7可以促进Ⅱ型胶原基因和TIMP-1基因表达。结论BMP-7可以对抗IL-6作用和正向调节椎间盘细胞Ⅱ型胶原基因及TIMP-1基因表达,提示BMP-7在临床治疗中可能具有逆转早期椎间盘退变的作用。

Objective To discuss the interaction of different concentrations of bone morphogenetic pmtein-7（BMP-7）and interleukin-6 （IL-6） on gene expression of type Ⅱ -collagen and TIMP-1 in the in vitro cultured human nucleus pulposus cells. Methods We used the RT-PCR semi-quantitative detection of the gene expression levels of type Ⅱ-collagen and TIMP-1 in the in vitro cultured human nucleus pulposus cells under the action of different concentrations of BMP-7 and IL-6. Results IL-6 reduced the gene expression levels of type II collagen and TIMP-1 ,and low concentration of BMP-7 could counteract the effects of the same concentration of IL-6 to protect the nucleus pulposus cells. High concentration of BMP-7 could promote the gene expression of type Ⅱ-collagen and TIMP-1. Conclusion BMP-7 can antagonize the effects of IL-6 and regulate positively the gene expression of type II collagen an d TIMP-1 in the nucleus pulposus cells, suggesting BMP-7 could reverse the degeneration of intervertebral discs in the early stage of clinical treatment.