摘要
目的了解三氧化二砷对胃腺癌SGC7901细胞株细胞增殖、细胞凋亡以及bcl-2、cyclin D1基因表达的影响。方法分别以不同浓度的三氧化二砷作用于SGC7901细胞,作用不同时间后采用MTT检测法对细胞增殖活性进行检测;采用流式细胞仪(FCM)AnnexinV/PI双染法检测细胞凋亡率;逆转录-聚合酶链式反应(RT-PCR)检测bcl-2、cyclinD1基因转录水平。结果稍高浓度(≥2μmol/L)的三氧化二砷可抑制SGC7901细胞增殖,并有剂量和时间依赖性。逆转录聚合酶链式反应(RT-PCR)检测bcl-2、cyclin D1基因转录水平,发现三氧化二砷作用组bcl-2、cyclin D1基因的表达与对照组比较明显减低(P<0.01)。结论三氧化二砷可有效抑制SGC7901细胞增殖,诱导SGC7901细胞凋亡,与作用剂量和作用时间呈正相关,这一过程可能与三氧化二砷抑制bcl-2、cyclin D1表达有关。
Objective To investigate the effect of arsenic trioxide on gastric adenocarcinoma cell line SGC7901 cell proliferation, apoptosis,and bcl-2,cyclin DI gene expression. Methods SGC7901 cells were treated with different concentrations of arsenic trioxide at different time and after that,the proliferation of SGC7901 cells,the apoptosis rate and the gene transcription level of bcl-2 and cyclin DI were detected by MTF,by flow cytome｜ry and AnnexinV/PI double staining and by RT-PCR,respectively. Results Slightly high concentration of arsenic trioxide（/〉2 μ mol/L） could inhibit cell proliferation and induce SGC-7901 apoptosis, with a dose-and time -dependency. The bcl-2 and cyclin D1 gene expression was obviously lowered in the arsenic trioxide group than that of the control （P〈0.01）. Conclusion Arsenic trioxide can effectively inhibit cell proliferation and induce SGC7901 apoptosis,with a direct relation to the dose and time,which may be correlated with the inhibition of bcl-2 and cyclin D1 expression by arsenic trioxide.
出处
《中国现代医生》
2010年第4期32-34,共3页
China Modern Doctor
