白血病细胞核基质蛋白的初步研究

Study on nuclear matrix protein in leukemia cells

目的比较白血病细胞与正常骨髓细胞核基质成分的差异。方法用高盐法抽取细胞核基质蛋白，ＳＤＳ－ＰＡＧＥ和ＷｅｓｔｅｒｎＢｌｏｔｔｉｎｇ分析和观察了急性粒细胞性白血病和慢性粒细胞白血病慢性期、急粒变期及化疗前后白血病细胞核基质蛋白的改变。结果慢性粒细胞白血病慢性期、急粒变期及急性粒细胞白血病细胞核基质与正常者有明显差异，主要改变是白血病细胞分别在某些区带出现新的核基质蛋白或核基质蛋白含量的增加，慢性粒细胞白血病急性变后与慢性期相比也在某些区带出现新的核基质蛋白或核基质蛋白含量的增加。化疗前后白血病细胞核基质蛋白成分的比较，表现为化疗后核基质蛋白明显减少。结论白血病细胞与正常骨髓细胞相比，核基质蛋白有明显的差异，核基质蛋白成分的改变可能与白血病的发生、发展以及慢性粒细胞白血病的急性变有关，核基质是某些抗癌药物的作用位点。

Objective To compare the composition of nuclear matrix proteins (NMP) between leukemia cells and normal bone marrow cells. Methods NMP was isolated by high salt extration and identified by SDS PAGE and western blotting. Results Increase in NMP was demonstrated in acute and chronic myelogenous leukemic cells as well as in the blast phase of chronic leukemia. On SDS PAGE, NMPs with molecular weight different from what were seen in normal bone marrow cells were present in both acute and chronic myelogenous leukemias. Conclusion Marked changes of NMP, not only in contents but also in compositions, exist in leukemic cells compared with normal bone marrow cells. NMP may serve as a target of chemotherapeutic drugs against leukemia.